WormMine: AnatomyTerm WBbt:0005833

• Definition: one of the two AWC neurons, stochastically determined, in which the str-2 promoter is inactive
• Name: AWC-OFF
• Primary Identifier: WBbt:0005833

0 Children

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in AWC_OFF according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:AWC_OFF_enriched

35 Expression Patterns

• Primary Identifier: Expr14133
• Remark: Strain: OH14774
• Pattern: Asymmetric expression in AWC OFF, ASK, ASI

• Primary Identifier: Expr15422

• Primary Identifier: Expr12697
• Pattern: The GPCR gene srsx-3 is expressed in the AWC-OFF neuron.

• Primary Identifier: Expr14145
• Pattern: Asymmetric expression in AWC OFF (dim and variable)

• Primary Identifier: Expr16432
• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15606

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr11487
• Remark: Temporal description
• Pattern: The die-1 fosmid reporter is initially expressed very broadly throughout the developing embryo, including the hypodermis, where die-1 acts to ensure ventral enclosure during gastrulation. Mirroring antibody staining, reporter expression of the fosmid reporter vanishes at approximately 460 min of embryonic development. die-1 expression then reinitiates in many neurons,including both ASEL and ASER; shortly thereafter, expression in ASER becomes undetectable. ASEL-restricted expression is maintained throughout the embryonic, larval, and adult stages. die-1 is also expressed in an asymmetric manner in the AWC olfactory neuron pair. Most adult animals show a strong bias of expression of die-1 to either the left or right AWC neuron. This antisymmetric die-1 expression is already evident in late embryos when the initial decision of AWC asymmetry control is made. ASEL-specific expression of die-1can be observed before any AWC expression of die-1 is observed. Expression of the die-1 fosmid reporter correlates with the AWC OFF state.

• Primary Identifier: Expr14175
• Remark: Other Strain: OH13866
• Pattern: Asymmetric expression in AWC OFF, ADL, 2 more dim pairs, head mesodermal cell, vulval muscle, anal depressor muscle

• Primary Identifier: Expr14226
• Remark: Other Strain: OH14395
• Pattern: Assymmetric expression in AWC OFF, a couple of dim neuron pairs

• Primary Identifier: Expr11623
• Pattern: srt-45 was expressed strongly in a single AWC neuron and weakly in an additional pair of head neurons. Expression was present in AWCOFF but not AWCON, based on coexpression with the srsx-3::mCherry reporter.

• Primary Identifier: Expr15648

• Primary Identifier: Expr15575

• Primary Identifier: Expr15596

• Primary Identifier: Expr10194
• Pattern: 52% of animals had visible mir-71p::YFP in both AWC cells, 28% had visible YFP in only AWC left (AWCL), and 20% had visible YFP in only AWC right (AWCR).bmir- 71p::GFP expression was significantly higher in the AWC-ON cell than in the AWC-OFF cell in 71% of the animals.

• Primary Identifier: Expr15376

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of two ciliated neurons with large, flattened, sheet-like endings that are associated with the sheath cells of the amphid sensilla.	AWC		WBbt:0005672