WormMine: ExpressionPattern Expr16432

• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.
• Primary Identifier: Expr16432

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASER	lineage name: ABpraaapppaa	WBbt:0003903
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGL	lineage name: ABalppappap	WBbt:0003824
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGL	lineage name: ABplaapapap	WBbt:0003892
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGR	lineage name: ABpraapapap	WBbt:0003891
Neuron, ciliated ending in head, no supporting cells, associated with ILso	BAGR	lineage name: ABarappppap	WBbt:0003823
posterior region, from rectum to the end	tail		WBbt:0005741
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBR	lineage name: ABpraaappap	WBbt:0003828
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBL	lineage name: ABalpppppap	WBbt:0003829
Amphid finger cell, neuron associated with amphid sheath	AFDR	lineage name: ABpraaaapav	WBbt:0003991
Amphid finger cell, neuron associated with amphid sheath	AFDL	lineage name: ABalpppapav	WBbt:0003993
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798
one of the two AWC neurons, stochastically determined, in which the str-2 promoter is active	AWC-ON		WBbt:0005832
one of the two AWC neurons, stochastically determined, in which the str-2 promoter is inactive	AWC-OFF		WBbt:0005833

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022060	dmd-9	Y67D8A.3	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The sixth sub-stage of the L4 larva. The side of the vulval lumen between vulC and vulD forms a smooth curve. At 20 Centigrade: 40.4 hours after hatching, 6.4 hours from the L3-to-L4 molt.		L4.5 larva Ce	WBls:0000688
	The fifth sub-stage of the L4 larva. utse is visible as a thin layer separating the vulval and uterine lumens. 'Fingers' are formed at the sides of the vulva next to vulB1 and vulB2. The uterine lumen is fully distended. At 20 Centigrade: 39.0 hours after hatching, 5.0 hours from the L3-to-L4 molt.		L4.4 larva Ce	WBls:0000687
	The tenth sub-stage of the L4 larva. The vulval lumen is completely collapsed. At 20 Centigrade: 42.8 hours after hatching, 8.8 hours from the L3-to-L4 molt.		L4.9 larva Ce	WBls:0000692
	The ninth sub-stage of the L4 larva. The vulval lumen is partially collapsed. At 20 Centigrade: 42.5 hours after hatching, 8.5 hours from the L3-to-L4 molt.		L4.8 larva Ce	WBls:0000691
	The eighth sub-stage of the L4 larva. vulFs have migrated closer such that the lumen is narrowed in the dorsal section. The approximate cutoff is when the width of the channel is less than the width of the vulD nucleus. At 20 Centigrade: 42.3 hours after hatching, 8.3 hours from the L3-to-L4 molt.		L4.7 larva Ce	WBls:0000690
	The seventh sub-stage of the L4 larva. 'Fingers' between vulB2 and vulC are pointed ventrally. At 20 Centigrade: 41.5 hours after hatching, 7.5 hours from the L3-to-L4 molt.		L4.6 larva Ce	WBls:0000689