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Expr12103
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Eggs already showed the corresponding GFP fluorescence, with more and more tissues following during development (L1 larval stage: pharynx, excretory cell, some sensory neurons; L2-L4 larval stages: intestine, rectal sensory neurons; adult worms: longitudinal muscles, gonads). The rare males showed GFP expression in their spermathecae. Within the anterior part of the nervous system, GFP expression was detected in the pharyngeal nerve ring and in some sensory neurons. Within its posterior part, lumbar ganglia and the dorso-rectal ganglion showed GFP expression. |
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Expr10755
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Previous studies have shown that this particular nlp-14 promoter sequence drives expression of GFP reporters to a subset of nervous system cells common to both sexes: the sensory neurons ASI, ASK, ASE, PHA, two retrovesicular ganglion neurons, ventral nerve cord motor neurons and the interneuron PVT. We find that in the male Pnlp-14::GFP reporters are additionally expressed in male-specific PVY, PVX and two male-specific dorsal rectal ganglion cells. |
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Expr1901
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The distribution of signal in transgenic worms was unique and highly restricted with respect to tissue and/or stage of development but did not correspond to the descendants of a particular branch of the cell lineage. Fluorescence was first detectable at the comma stage in cells that divided and appeared to migrate during the 2-fold and 3-fold stages. Neuronal staining was obvious from L1 onward and by early L4 was seen to occur in both the dorsal and ventral nerve chords. During this stage, a strong signal was noted in the developing vulva (most likely the vulE and/or vulF cells). By late L4 an intense GFP signal in the spermathecal valve as well as other vulval and/or uterine structures was evident. Expression in the uv1 and uv2 cells was suggested by the pattern of fluorescence around the vulva. However, the nuclear-localized reporter construct stained more nuclei than can be accounted for by expression in these cells alone. With this construct, nuclear localized signal was observed in all four nuclei of the syncytial spermathecal valve cell. Although GFP fluorescence was seen to be strongest in the late L4 and early adult for the spermathecal valve and vulval/uterine structures previously noted, it was seen to persist throughout adulthood. The M8 cell of the terminal bulb of the pharynx, all six cells of the pharyngeal-intestinal valve, and neuronal cell bodies within the metacorpus and around the isthmus of the pharynx also expressed gly-2p::GFP. At least 37 neurons with cell bodies lying next to the ventral nerve chord were positive for gly-2-directed reporter expression in the adult hermaphrodite, although with widely varying levels of staining. There was also GFP fluorescence present in other neurons associated with the preanal, dorso-rectal, and/or lumbar ganglia. In adult males, expression was similar in non-sexually dimorphic tissues and was also observed in axons that project into rays 2, 3, 5, 6, and either 8 or 9 of the copulatory bursa. |
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Expr1618
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A high proportion of PKC2 polypeptides is associated with the particulate fraction in newly-hatched (early L1) C. elegans and L3 and L4 larvae. |
In mid-L1 and L2 larvae, as well as young adult nematodes, PKC2 isoforms are nearly uniformly dispersed between cytosol and organelles and/or cytoskeleton. In contrast, 70% of PKC2 isoforms is isolated in cytosol from egg-laying adults. |
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Expr3254
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Although expression of the abts-2::GFP transcriptional fusion was restricted to a single cell of the rectal ganglion, the abts-2 translational fusion was strongly expressed in the excretory cell of larva. In addition, the ABTS-2::GFP fusion was expressed in the adult ovaries. |
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