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Expr9855
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LET-526::GFP was expressed in the nuclei of most, if not all, somatic cells, including the T cell and its daughter cells. LET-526::GFP was first observed soon after the gastrulation stage and continues to be expressed throughout subsequent development and in the adult stage. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr669
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EGL-43 is nuclear localized and is expressed in HSN before and during the cells migration from tail to the gonad primordium of the embryo. EGL-43 expression is down regulated after HSN migration. In embryo ~400 min after cleavage, expression observed in HSN/PHB precursor, PHA sensory neuron in the tail. ~430 min after first cleavage, expression observed in HSN nucleus which has migrated out of the tail, as well as a phasmid neuron nucleus. During its migration, HSN expresses EGL-43 as well as phasmid neuron nuclei. |
Expressed in the nuclei. |
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The alr-1p::GFP reporter construct was generated by inserting 1 kb of upstream regulatory sequence into the pPD95.75 vector, which contains the green fluorescent protein (GFP) coding sequence and the 3' untranslated region of unc-54 at the 3' end. This construct was injected into N2 worms at 10 ng/ul along with a PCR product corresponding to 6 kb of overlapping alr-1 upstream regulatory sequence and a dominant Roller marker, pRF4 containing rol-6 (su1006),at 100 ng/ul to generate kuEx146. |
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Expr3525
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Embryonic analysis indicated an early expression pattern just after the 28-cell stage. The strongest expression at this point was seen in descendants of the C linage as well as less prominent expression within a subset of the AB lineage. By the comma stage (-400 cells), GFP expression was apparent in alternating dorsal hypodermal cells before the onset of cell fusions. After the onset of the hypodermal cell fusions, GFP was apparent throughout the hyp7 hypodermal syncytium. At the comma stage, GFP was also strongly expressed in the precursors to the PLM and ALN neurons, the T-cells (precursors to the phasmid socket cells), and the cells that would comprise the hyp4 anterior hypodermal syncytium. These cells were tentatively identified based on cell position and the strong expression seen within the adult structures derived from these cells. A number of GFP-expressing cells within the head region of the embryo remained unidentified. These cells likely include a number of head and pharyngeal neurons, although other cells types are not ruled out. Expression in the larval and adult hermaphrodite was primarily restricted to a subset of neurons and neuronal support cells. The PLM, ALM, and AVM touch neurons and the intrinsic pharyngeal neurons I2 and I6 all showed very strong expression throughout all larval stages and adulthood. The RIS neuron and one other unidentified, unpaired neuronal cell body located in the retrovesicular ganglion occasionally showed faint expression. Strong expression was also seen in the glial-like amphid and phasmid socket cells (AMso and PHso 1 and 2) throughout larval development and adulthood. Strong GFP expression was also observed within the hyp6 and hyp4 hypodermal syncytia, the distal most segments of the intestine and the coelomocytes, the scavenger cells located within the pseudocoelom. Variable GFP expression was also seen in larval and adult worms within the hyp7 hypodermal syncytium. Importantly, GFP expression was not observed in 23 of the 24 GABAergic neurons (the sole exception being the RIS neuron) that have been shown previously to stain with anti-ALR-1 antibodies (See Expr3487). This suggested that the sequences required for ALR-1 expression in these neurons may reside outside of the 6 kb of promoter sequence driving this GFP reporter. Alternatively, GFP expression from this construct may not be readily detectable above background fluorescence in these specific neurons. |
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Expr9857
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In wild-type animals, psa-3 was expressed in the T cell; after its division, it was expressed more highly in the T.p cell than in the T.a cell. |
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Expr9858
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In wild-type animals, tlp-1 was expressed in the T cell; after its division, it was expressed more highly in the T.p cell than in the T.a cell. |
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[PBRM-1::gfp] translational fusion. The pbrm-1::gfp construct consisted of a genomic fragment from a cosmid C09B10 (a 2-kb fragment from 1.5-kb upstream of the start codon to the XbaI site) and a PCR fragment (XbaI site to the codon that corresponds to the C-terminus of PBRM-1). which was amplified from the yk1173e11 cDNA clone (a gift from Y. Kohara, National Institute of Genetics, Mishima, Japan) and ligated to the gfp gene from pPD95.75 (a gift from A. Fire). |
Expr9856
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PBRM-1::GFP was expressed in the nuclei of most, if not all, somatic cells, including the T cell and its daughter cells. PBRM-1::GFP was first observed during the gastrulation stage and continues to be expressed throughout subsequent development and in the adult stage. During mitosis, PBRM-1::GFP was associated with chromosomes in the T cell and in other seam cells. |
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Expr11089
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ceh-20 was expressed in nearly all of the cells, including the T cell. During T cell mitosis, CEH-20 was distributed uniformly. Soon after T cell division (before V6 division), the nuclear level of CEH-20 was equal in the daughter cells, while in the late stage, after V6 division, the nuclear level in T.a was slightly greater and that in T.p was slightly less, resulting in the differential subcellular localization of CEH-20 between the daughter cells (8/33 animals showed the asymmetric localization; i.e., strong nuclear localization in T.a and weak nuclear and cytoplasmic localization in T.p). |
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Expr12800
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nhr-25 was expressed in V seam cells, in the posterior seam-cell T and its daughters (T.a, T.p), and in the hyp cells of wild-type animals. |
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