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Expr1569
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Weak expression of lin-17 detected in the vulva cells that form the vulva only after the vulva precursor cells had divided twice, i.e., in all Pn.pxx cells. lin-17 expression was also observed in the male tail throughout development, with the expression strong particularly at later L3 stage in most descendants of the V6 and T cells in the lateral hypodermis. lin-17 was detected in the P11.p and B cells. This expression was almost uniform around the cell surface prior to their asymmetric divisions. Lin-17 was also detected in both daughters and all the granddaughter cells of the P11.p and B cells. Expression in the daughter cells were also uniform around the cells. In addition, weak lin-17 expression was detected in the P10.p cell and both of its daughter cells. Authors failed to detect lin-17 expression in P7.p, Z1, Z4 and T cells. |
Expressed on cell membrane. |
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Expr2276
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Neuronal expression. In the L1 stage cog-1::gfp is expressed in amphid neurons ADL(L/R), ASE(L/R), and ASJ(L/R). One additional pair of head neurons expresses cog-1::gfp in the L1. This pair is located between the excretory cell and the excretory pore, and is probably either AIA(L/R), SMBD(L/R), SIAD(L/R), or SIAV(L/R). In the tail, phasmid neurons PHB(L/R) express cog-1::gfp. The expression in amphid and phasmid neurons persists to the adult. Additional unidentified cells in the preanal ganglion express cog-1::gfp in late larvae and adults. Other cells in the tail region. The sphincter muscle (mu_sph) and phasmid sheath cells (PHshL, PHshR) express cog-1::gfp in all stages examined. Uterine expression. In the hermaphrodite gonad, cog-1::gfp is expressed exclusively in the dorsal uterine lineage. During the L3 stage, GFP is in the central four great-granddaughters of DU cells (DE4/DE5; DE, dorsal eight). The expression apparently persists in DE4/DE5 descendants, du, uv2, and uv3, until the adult stage, DE2 and DE7 lineages in the dorsal uterus also express cog-1::gfp starting from the early L4 stage. In the late L4, cog-1::gfp in this region is observed exclusively in sujc cells. Each core is surrounded by the spermathecaluterine valve and appears to form a plug which blocks the uterine lumen from the spermathecal lumen. None of the cog-1::gfp fusions, including the rescuing construct, was ever observed to express in any ventral uterine cells, including the anchor cell. Thus, the function of cog-1 in the connection is believed to be a consequence of abnormal gene regulation in the vulva, although a function in the dorsal uterine cells have not been ruled out. Vulval expression. In the vulva, cog-1::gfp is expressed from early L4 to the adult. Cells that express are vulC [P5.ppa(l/r), P7.pap(l/r)], vulD (P5.ppp, P7.paa), vulE(P6.pppl, P6.pppr, P6.paal, P6.paar), and vulF (P6.ppal, P6.ppar, P6.papl, P6.papr). The expression is considerably brighter in vulC and vulD than in vulE and vulF. Occasionally, expression in vulB and vulA was observed. Male expression. The expression of cog-1::gfp in the male was scored mostly in animals containing an extrachromosomal array containing the SmaI insertion. cog-1::gfp is expressed during proctodeal development. eL.aav and eR.aav express cog-1. In addition, proctodeal cells B.pap and B.pppa express cog-1::gfp. Additional expressing cells observed in occasional males include: rep, and P11.pp progeny. No gonadal cells in the male, including the linker cell, expressed cog-1::gfp in any stage of development. |
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Expr1516
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About 8 hour after hatching, P cells descend into the ventral nerve cord and interdigitate to form a single row of cells of P1-P12. In both sexes, mab-5 was detected in P7-P10. In older larva, mab-5 was detected in P7-P12, but not more anterior cells. In hermaphrodites, expression pattern was similar to males with two exceptions. The p(9-11).app cells and p(9-11).p cells no longer express MAB-5 by L2. In male, descendants of P10 consistently express high levels of MAB-5, those of P9 and P11 express low levels, and those of P7 and P8 express barely detectable levels. P12 descendants express MAB-5 only until the division of P12.a. The descendants of P cells express comparable levels of MAB-5 with exceptions to Pn.p cells, they express lower levels than their neighbors. Expression levels in P descendants were similar to those seen in the neighboring juvenile motoneurons. Both sets of cells expressed MAB-5 in a graded pattern, tapering off toward the anterior. In newly hatched larva, in both sexes, mab-5 was detected consistently in nuclei of P9/10 and P11/12, but not more anterior P cells. mab-5 was also detected at high levels in the posterior juvenile motoneurons, located ventrally. MAB-5 is localized to the posterior of the body, including P7-P12 in the P lineage but not in more anterior cells. Within P7-P12, MAB-5 expression was graded with the highest levels in the P10 descendants. The descendants of any one P cell expressed comparable levels of MAB-5. |
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