WormMine: AnatomyTerm WBbt:0004603

• Definition: Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor
• Name: VD4
• Primary Identifier: WBbt:0004603
• Synonym: lineage name: P3.app

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree P3.app	P3.app nucleus		WBbt:0003233

2 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in VD_DD according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VD_DD_enriched
	Transcripts enriched in VD3_7 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00066550:VD3_7_enriched

46 Expression Patterns

• Primary Identifier: Expr15619

• Primary Identifier: Expr15558

• Primary Identifier: Expr15560

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr13158

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr1734
• Remark: Lines expressing GFP::CED-10 were generated by injection of ced-10::gfp::ced-10 with an unc-76-rescuing construct, P76-16B, both at 75 ng/l, into unc-76(e911) animals.
• Pattern: Cell type identifications were confirmed by the examination of a nuclear-localized GFP expressed from the ced-10 promoter. GFP::CED-10 was expressed broadly, perhaps in all cells, including neurons (CANs, VDs and DDs), engulfing cell types (including the hypodermis, intestine and pharynx) and the distal tip cells. Expression was first seen in early embryogenesis and continued throughout adulthood in all cell types.
• Subcellular Localization: Strong fluorescence was observed within the axons of the nerve ring. The GFP::CED-10 fusion protein accumulated at the plasma membrane.

• Primary Identifier: Expr15570

• Primary Identifier: Expr15644

• Primary Identifier: Expr15648

• Primary Identifier: Expr15641

• Primary Identifier: Expr15633

• Primary Identifier: Expr15335

• Primary Identifier: Expr13159

• Primary Identifier: Expr16257
• Pattern: For lin-39 and mab-5, previous work reported their partially overlapping expression in the ventral cord motor neurons (MNs). In this study, we used two fosmid reporters wgIs18[lin39::EGFP] and wgIs27[mab-5::EGFP] to map their expression to the single-cell resolution among the 75 motor neurons (MNs), which include 54 cholinergic MNs, 19 GABAergic MNs, and 2 serotonergic MNs with stereotypical positions. These MNs can be further classified into eight neuron classes (DA, DB, VA, VB, AS, VC, DD, and VD). By crossing the GFP reporters with mCherry strains labelling cholinergic or GABAergic neurons in the ventral cord, we identified lin-39 expression in DA2-5, DB2-7, VA3-8, VB4-9, AS2-8, VC1-6, DD2-6, and VD3-12 and mab-5 expression in DA4-8, DB5-7, VA6-11, VB8-11, AS5-11, VC3-6, DD2-6, and VD2-12. Both lin-39 and mab-5 expression were generally weaker in more anterior MNs. Interestingly, for cholinergic MNs, mab-5 and lin-39 expression only overlapped in a set of mid-body MNs; MNs more anterior to this region expressed only lin-39 and MNs more posterior expressed only mab-5. For GABAergic MNs (DD and VD), however, lin-39and mab-5 expression overlapped entirely. Outside of the ventral nerve cord, lin-39 was expressed in AQR and AIYL/R in the head and AVM, SDQL/R, PDEL/R, and PVDL/R neurons along the body; mab-5 was expressed in the AVL in the head, SDQL in the mid-body region, and PQR neuron in the tail.

• Primary Identifier: Expr15628

• Primary Identifier: Expr1459
• Remark: Suncellular localization was revealed by another construct with GFP inserted at the carboxy terminus of the UNC-47 protein. This construct rescued the unc-47 mutant phenotype, demonstrating that the construct functions normally.
• Pattern: Animals containing the unc-47::GFP reporter construct showed expression of GFP in all GABAergic neurons, and only in GABAergic neurons.
• Subcellular Localization: Done by another gfp construct: GFP-tagged UNC-47 was localized to synaptic varicosities along the ventral and dorsal cords but not in axons. show that unc-47 expression is associated with synaptic vesicle.

• Primary Identifier: Expr1205
• Remark: Reporter gene fusion type not specified. These expression patterns were identical to those for GABA, unc-47, and unc-25.
• Pattern: expressed in all GABAergic neurons, which include the 19 D neurons in the ventral cord, the four RMEs, AVL, and RIS in the head, and DVB in the tail.

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
Neuron class of thirteen motoneurons, with cell bodies in the ventral cord, which innervate ventral muscles. Each cell has an anteriorly directed process emanating from its cell body.	VD neuron		WBbt:0005303
post-embryonic cell of pedigree P3.ap	P3.ap		WBbt:0007242