WormMine: AnatomyTerm WBbt:0004965

• Definition: Sensory neuron of copulatory spicle in male, cilliated, open to outside at tip of spicule
• Name: SPDR
• Primary Identifier: WBbt:0004965
• Synonym: lineage name: B.arpapaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree B.arpapaa	B.arpapaa nucleus		WBbt:0002840

0 Expression Clusters

9 Expression Patterns

• Primary Identifier: Expr11704
• Pattern: Expression of the transcriptional fusion Ptop-1::gfp was not detected in early embryonic cells due to maternal germline silencing of the multi-copy transgenic gene. The GFP expression, however, was observed in most cells at the late embryonic stage and decreased with larval development. In the larval stages, GFP expression was predominantly present in the neuronal system, including sensory neurons (ILso, URX, RIC, IL1/IL2, AIY/AIM and RIG/RIF), motor neurons (VC4, VC5, HSN, PVD and PVM) of hermaphrodites and tail neurons (SPD and SPV/SPC) of males. The expression of the transcriptional fusion Ptop-1::gfp was strong in DTCs during the L3-L4 stage when gonad migration proceeds.

• Primary Identifier: Expr15370

• Primary Identifier: Expr296
• Remark: Data modified according to Shawn Lockery's expression pattern curations.
• Pattern: ASIf ASH SPDm/SPVm PVQ

• Primary Identifier: Expr12263
• Pattern: In contrast to hermaphrodites, lgc-35 is not expressed in any of the male enteric muscles, including the sphincter muscle, suggesting that it does not play a role in defecation in adult males. Like hermaphrodites, adult males expressed lgc-35 in acetylcholine motor neurons, the head mesodermal cell, and in the head interneurons AIY and AVD. In the male tail, lgc-35 is expressed in sensory rays 2, 3, 4, 5, and 9, the oblique muscle, the SPV neurons, and possibly the SPD neuron.

• Primary Identifier: Expr950
• Pattern: Early in development, 1497pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. Early in development, pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites.

• Primary Identifier: Expr522
• Remark: syIs20[gpa-1::lacZ dpy-20+]
• Pattern: Expressed in SPD cells and phasmids at adult. In head region also.

• Primary Identifier: Expr15831
• Pattern: Expression analysis of a FLP-3 translational fusion (pflp-3::flp- 3::mCherry) confirmed previous expression analyses of the neuropeptide within male-specific spicule neurons. Transcriptional reporters have shown robust flp-3 expression in the amphid IL1 neurons, as well as the sensory PQR and the male-specific interneuron, CP9, although our translational fusion exhibited no PQR or CP9 expression. Previous studies employed 1-2kb regions of promoter sequence driving GFP expression,while our construct employs a 4 kb region, thereby incorporating further regulatory elements that may restrict expression patterns. By including the full coding sequence in our translational fusion, we have also incorporated the regulatory elements found within the introns of the flp-3 gene.Interestingly, we observed localization of mCherry within sensory cilia of male dorsal and ventral IL1 neurons, as well as in puncta spanning their dendrites, consistent with peptide packing into dense core vesicles. Previous studies have observed dense core vesicles within, and being released from, dendritic arbors, supporting our findings that FLP-3 is packaged within the dendrites of the IL1 neurons. We found an identical expression pattern within the IL1 cilia and dendritic puncta of hermaphrodites as well. In males, our flp-3 construct also exhibited male-specific tail expression in the SPD spicule neurons.

• Primary Identifier: Expr15030
• Pattern: In addition to male sex muscles, EGL-2::YFP is also expressed on the cell bodies, neural processes, and sensory endings of the male ray, post-cloacal, and spicule sensory neurons. the protractor muscles' EGL-2::YFP levels also increased between 24 and 48 h in wild-type males.

• Primary Identifier: Expr293
• Remark: Data modified according to Shawn Lockery's expression pattern curations.
• Pattern: SPD/SPV, males only.

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage after an male animal is fully-developed and reaches maturity.		adult male Ce	WBls:0000056

3 Parents

Definition	Name	Synonym	Primary Identifier
post-embryonic cell of pedigree B.arpapa	B.arpapa		WBbt:0007071
Neuron class of two male-specific sensory neuron associated with copulatory spicules, cilliated, open to outside at tip of spicule.	SPD		WBbt:0007805
	copulatory spicule right		WBbt:0005314