WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Life Stage :

Definition  The stage after an male animal is fully-developed and reaches maturity. Primary Identifier  WBls:0000056
Public Name  adult male Ce
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6 Anatomy Terms

Definition Name Synonym Primary Identifier
Male specific sensory /motor neuron, innervates spicule protractor muscle SPCL lineage name: B.alpaap WBbt:0004968
Male specific sensory /motor neuron, innervates spicule protractor muscle SPCR lineage name: B.arpaap WBbt:0004967
Sensory neuron of copulatory spicle in male, cilliated, open to outside at tip of spicule SPDR lineage name: B.arpapaa WBbt:0004965
Sensory neuron of copulatory spicle in male, cilliated, open to outside at tip of spicule SPDL lineage name: B.alpapaa WBbt:0004966
Sensory neuron of copulatory spicle in male, cilliated, open to outside at tip of spicule, of pedigree B_beta.rda. SPVR B_beta.rda WBbt:0004963
Sensory neuron of copulatory spicle in male, cilliated, open to outside at tip of spicule SPVL lineage name: B.al/raalda WBbt:0004964

1 Contained In

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041

45 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts that showed significantly increased expression in mrg-1(qa6200) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mrg-1(qa6200)_upregulated_PGCs
  male soma-enriched Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. [cgc6390]:male_soma-enriched
  Transcripts expressed in male vas deferens, according to RNA tomography. RNA tomography WBPaper00055648:male-vas-deferens_expressed
  Transcripts expressed in spermatheca, according to RNA tomography. RNA tomography WBPaper00055648:spermatheca_expressed
  Transcripts that showed significantly decreased expression in mes-3(bn199) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-3(bn199)_downregulated_PGCs
  Transcripts that showed significantly decreased expression in mrg-1(qa6200) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mrg-1(qa6200)_downregulated_PGCs
  Transcripts expressed in germline, according to RNA tomography. RNA tomography WBPaper00055648:germline_expressed
  Transcripts expressed in male tail, according to RNA tomography. RNA tomography WBPaper00055648:male-tail_expressed
  Transcripts enriched in male him-5(e1490 animals, comparing to in N2 hermaphrodite animals from L3 larva to young adult stages. DESeq FDR < 0.01. WBPaper00050231:male_enriched
  Transcripts expressed in male CEM neurons, according to RNA tomography. RNA tomography WBPaper00055648:male-CEM-neurons_expressed
  Transcripts that showed significantly increased expression in mes-3(bn199) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-3(bn199)_upregulated_PGCs
  Transcripts that showed significantly increased expression in mes-4(bn73) comparing to in control animals in early germ cells (EGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-4(bn73)_upregulated_EGCs
  Transcripts expressed in male seminal vesicle, according to RNA tomography. RNA tomography WBPaper00055648:male-seminal-vesicle_expressed
  Genes that showed increased expression in alg-3/4 males comparing to in WT males, according to RNAseq results. More than 2 fold change in expression were considered differentially expressed. WBPaper00044616:alg-3-4_upregulated_gene
  male sex-enriched Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. [cgc6390]:male_sex-enriched
  Transcripts expressed in anterior head region, according to RNA tomography. RNA tomography WBPaper00055648:anterior-head_expressed
  Transcripts expressed in pharynx, according to RNA tomography. RNA tomography WBPaper00055648:pharynx_expressed
  Transcripts expressed in sperm, according to RNA tomography. RNA tomography WBPaper00055648:sperm_expressed
  Transcripts expressed in nerve ring, according to RNA tomography. RNA tomography WBPaper00055648:nerve-ring_expressed
  male germline-enriched Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. [cgc6390]:male_germline-enriched
  Transcripts that showed significantly decreased expression in mes-4(bn73) comparing to in control animals in early germ cells (EGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-4(bn73)_downregulated_EGCs
  Transcripts that showed significantly increased expression in spe-44(fx110) comparing to in control male animals. DESeq2, fold change > 2 and a BenjaminiHochberg multiple hypothesis-corrected P-value cutoff of 0.05 was used as a cutoff for significant differen-tial expression. WBPaper00064502:spe-44(fx110)_upregulated
  Transcripts that showed significantly decreased expression in nhr-23(kry61);spe-44(fx110) comparing to in control male animals. DESeq2, fold change > 2 and a BenjaminiHochberg multiple hypothesis-corrected P-value cutoff of 0.05 was used as a cutoff for significant differen-tial expression. WBPaper00064502:nhr-23(kry61);spe-44(fx110)_downregulated
  Transcripts that showed significantly decreased expression in mes-4(bn73) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-4(bn73)_downregulated_PGCs
  Transcripts that showed significantly increased expression in mes-3(bn199) comparing to in control animals in early germ cells (EGCs) at L1 larva stage. DESeq2(v1.32.0), FDR < 0.05. WBPaper00064315:mes-3(bn199)_upregulated_EGCs
  Transcripts enriched in N2 hermaphrodite animals, comparing to in male him-5(e1490) animals from L3 larva to young adult stages. DESeq FDR < 0.01. WBPaper00050231:hermaphrodite_enriched
  Transcripts that showed significantly increased expression in nhr-23(kry61);spe-44(fx110) comparing to in control male animals. DESeq2, fold change > 2 and a BenjaminiHochberg multiple hypothesis-corrected P-value cutoff of 0.05 was used as a cutoff for significant differen-tial expression. WBPaper00064502:nhr-23(kry61);spe-44(fx110)_upregulated
  Transcripts that showed significantly increased expression in nhr-23(kry61) comparing to in control male animals. DESeq2, fold change > 2 and a BenjaminiHochberg multiple hypothesis-corrected P-value cutoff of 0.05 was used as a cutoff for significant differen-tial expression. WBPaper00064502:nhr-23(kry61)_upregulated
  Transcripts that showed significantly decreased expression in spe-44(fx110) comparing to in control male animals. DESeq2, fold change > 2 and a BenjaminiHochberg multiple hypothesis-corrected P-value cutoff of 0.05 was used as a cutoff for significant differen-tial expression. WBPaper00064502:spe-44(fx110)_downregulated
  Proteins expressed in activated sperm fraction after C. remanei spermatids were activated in vitro. N.A WBPaper00054996:activated_sperm_CRE

3 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr13253 Adult males exhibit daf-7 expression in both the ASI and the ASJ neuron pairs in the absence of P. aeruginosa. This expression pattern difference in adult C. elegans, can be observed both through the use of a pdaf-7::gfp transcriptional reporter as well as by fluorescence in situ hybridization (FISH) that directly probes for endogenous daf-7 mRNA. In populations of early larval animals carrying the pdaf-7::gfp transcriptional reporter, males and hermaphrodites were indistinguishable, with fluorescence observed only in the ASI neurons. To determine the onset of daf-7 expression in males, we imaged animals carrying the fluorescent reporter at multiple time points during development beginning in the L4 larval stage and extending into reproductive maturity. We found that daf-7 expression remains off in the ASJ of males through the end of the last (L4) larval stage, but is switched on during young adulthood as the animals reach reproductive maturity. In addition, we examined expression of daf-7 in the ASI neuron pair at several matched timepoints during development and observed a significant drop in the expression of daf-7 in the ASI neurons from L4 to young adulthood (52 hr and 64.5 hr timepoints). At subsequent timepoints during adulthood, however, expression levels in the ASI neurons remained constant as expression in the ASJ neurons increased (64.5 hr and 71.5 hr timepoints).  
Reporter gene fusion type not specified.   Expr1148 A 6.7-kb fusion (LOV-1::GFP1) and a 15.4-kb fusion lacking only 58 amino acids (LOV-1::GFP5) direct expression in male-specific sensory neurons. Shorter versions of lov-1::gfp1 are not expressed in the same set of male-specific neurons, nor do they act as dominant negatives. lov-1::gfp1 and lov-1::gfp5 are expressed in male-sensory neurons, specifically the CEM neurons, the hook neuron HOB, and the sensory ray neurons. Expression begins during late L4 lethargus and peaks in the adult male. LOV-1::GFP1 is localized at high levels in the cell body (punctate cytoplasmic expression) and ciliated endings, whereas LOV-1::GFP5 is found exclusively in cell bodies.
    Expr13283 mRNA levels of both cyb-1 and cyb-3 were high in young-adult and adult N2 hermaphrodites as well as in feminized fem-1(hc17lf) adult hermaphrodites. By contrast, cyb-1 mRNA, but not cyb-3 mRNA, was highly expressed in the L4 stage N2 hermaphrodites, adult N2 males, and masculinized fem-3(q20gf) adult hermaphrodites, in all of which spermatogenesis proceed specifically and robustly. On the other hand, mRNA levels of both cyb-1 and cyb-3 were negligible in germline proliferation-defective glp-1(q224lf) adult hermaphrodites. These results indicate that cyb-1 is the major B-type cyclin robustly expressed during spermatogenesis.  

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0 Preceded By

0 Sub Stages