1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001333 | erm-1 | C01G8.5 | Caenorhabditis elegans |
| Primary Identifier | Expr2996 | Remark | Three different methods were used to show that the antiserum is specific for ERM-1. After RNAi depletion of ERM-1, immunological detection was greatly reduced or eliminated in embryonic extracts as well as in whole mount embryos. In addition, if whole mount WT embryos were incubated with an aliquot of antibodies that was preincubated with an excess of ERM-1 fusion protein before its use, no pattern of staining could be detected. |
| Subcellular Localization | In wild type embryos, the first anti-ERM-1 staining was detect in the two-cell stage highlighting the cell cortex below contacting plasma membrane domains. This subcellular localization persists in most cells for the next rounds of division. At the onset of morphogenesis (lima bean stage), several tissues develop a polarized phenotype and ERM-1 becomes enriched at the apical cell cortex of the hypodermis and the pharyngeal-intestinal primordium. At the tadpole stage, the intestinal cells have fully polarized and feature an apical membrane domain that is marked by the co-localization of ERM-1 and the intermediate filament protein IFB-2. The apical membrane domain is separated from the basolateral membrane domain by AJM-1 expression, which marks the AJ. There is little or no overlap between ERM-1 and AJM-1 staining; however, ERM-1 co-localizes with apically enriched F-actin. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001333 | erm-1 | C01G8.5 | Caenorhabditis elegans |
