| Primary Identifier | Expr15225 | Subcellular Localization | Live imaging of embryos reveals diffuse cytoplasmic expression of SIMR-1 in all stages, similar to previous observations of MUT-16 expression (Uebel et al. 2020). Because SIMR foci are present in the germlines of adult hermaphrodites and localize adjacent to P granules, we focused our analysis on the germline blastomeres and progenitor germ cells of embryos. In the 2-cell embryo, P granules segregate to the posterior P1 germline blastomere, yet no punctate SIMR foci are present. Similarly, no SIMR foci are found in 8-cell embryos as P granules begin associating with nuclear pores in the P3 germline blastomere. The 28-cell embryo yields the first observable SIMR focus adjacent to perinuclear P granules in the P4 germline blastomere. While we consistently observe SIMR foci in 28- to 50-cell embryos (n = 3), these foci are few and faint. Around the 100-cell stage, the P4 cell gives rise to the Z2 and Z3 PGCs, and it is here that we reliably observe bright and numerous SIMR foci. These bright foci also persist in the Z2/Z3 of late-stage embryos of 300 or more cells. Our data reveals the previously unknown embryonic appearance of SIMR foci. Consistent with their localization in adult germ cells, embryonic SIMR foci appear adjacent to both P granules and Mutator foci. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00015504 | simr-1 | C06A5.6 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| All C. elegans development stages, including embryo, larva and adult stage. | all stages Ce | WBls:0000002 |
