WormMine: ExpressionPattern Expr12999

• Pattern: The UNC-130 signal was first detectable in bean stage embryos (360 min after first cleavage at 22°C) in almost all ventral BWMs; much later than previously reported (Nash et al., 2000). No UNC-130 signal was detected in any dorsal BWM or its precursor during embryogenesis. By the 1.5-fold stage of embryogenesis (460 min) when differentiation is underway, the UNC-130 signal was clearly visible in all ventral BWMs with the exception of one or two left-right pairs located at the anterior end, consistent with unc-129 expression patterns previously described and discussed below ( Colavita et al., 1998). The anterior half of ventral BWMs consistently had a stronger superGFP signal than their posterior counterparts even though UNC-120 antibody signals were uniform among all BWMs. Confidence that the genome edited superGFP strain reflected endogenous unc-130 expression came from observations that the ventral BWM pattern completely overlapped (and extended) the BWM pattern obtained using rat polyclonal antibodies raised against the UNC-130 protein, UNC-130::GFP and UNC-130::mCh translational fusion reporter expression patterns, and unc-130::gfp transcriptional reporter patterns that were all observed predominantly in mid-body, often MS-derived ventral BWMs. Post-embryonically, the signal from the superGFP-edited allele was too faint in BWMs to detect in live animals, mimicking weak antibody staining that was observed in young larvae and early larval signals from the unc-130::gfp transcriptional reporters.
• Primary Identifier: Expr12999

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
body wall muscle cell on the ventral side of animal.	ventral body wall muscle		WBbt:0008390

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006853	unc-130	C47G2.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016