1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000846 | cup-5 | R13A5.1 | Caenorhabditis elegans |
| Primary Identifier | Expr2919 | Remark | Type: BSA-Rhod uptake in coelomocytes expressing a rescuing GFP::CUP-5 fusion protein. |
| Subcellular Localization | After 15 min of uptake, most of the BSA-Rhod is in compartments that mostly lacked GFP::CUP-5. These late endosomes are the ones that are normally labeled with RME-8::GFP. However, GFP::CUP-5 colocalized with 30% (n=30) of the BSA-Rhod budding from these compartments; indeed, GFP::CUP-5 localized to the limiting membrane of these nascent lysosomes. Almost all of the internalized BSA-Rhod colocalized with the GFP::CUP-5 on mature lysosomes 60 min (90%, n=100) or 24 h (100%; n=100) after the start of the uptake assay. These results indicate that CUP-5 localizes to nascent and mature lysosomes. In agreement with the BSA-Rhod time courses in both RME-8::GFP and GFP::CUP-5 expressing coelomocytes, most of the RME-8 and CUP-5 proteins localize to different compartments. However, small discrete domains of CUP-5 localization on RME-8-labeled compartments were consistently observed. These results indicate that CUP-5 is found on subdomains of the late endosomes. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000846 | cup-5 | R13A5.1 | Caenorhabditis elegans |
