| Pattern | BAR-1-GFP expression first appeared in P11.p in the late L1 stage. In the early-to-middle L2 stage, BAR-1-GFP accumulated in the cytoplasm of P11.p in a punctate pattern, presumably resulting from the stabilization of BAR-1 in response to increased Wnt signaling. The punctate GFP fluorescence in the cytoplasm of P11. p rapidly decreased during the mid-to-late L2 stage. By the mid-L3 stage, just before P11.p divides, BAR-1-GFP expression appeared to be brighter in the nucleus than in the cytoplasm. The switch of cytoplasmic-to-nuclear BAR-1-GFP accumulation is initiated in the mid-to-late L2 stage, coincident with the time window critical for the specification of HCG cell fates. BAR-1-GFP expression was undetectable in P10.p prior to cell division but became visible in the nucleus of the posterior daughter, P10.pp, suggesting that Wnt signaling through BAR-1 likely acts during fate execution of some descendants of the P10.p lineage. Although we did not observe lin-17::GFP expression in P9.p, faint, mostly cytoplasmic expression of BAR-1-GFP was sometimes seen in P9.p up to the mid-L2 stage, just before P9.p fuses with hyp7. | Primary Identifier | Expr12038 |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| descendent cell of P11, posterior | P11.p | WBbt:0006899 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000238 | bar-1 | C54D1.6 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. | L2 larva Ce | WBls:0000027 |
