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Expr1569
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Weak expression of lin-17 detected in the vulva cells that form the vulva only after the vulva precursor cells had divided twice, i.e., in all Pn.pxx cells. lin-17 expression was also observed in the male tail throughout development, with the expression strong particularly at later L3 stage in most descendants of the V6 and T cells in the lateral hypodermis. lin-17 was detected in the P11.p and B cells. This expression was almost uniform around the cell surface prior to their asymmetric divisions. Lin-17 was also detected in both daughters and all the granddaughter cells of the P11.p and B cells. Expression in the daughter cells were also uniform around the cells. In addition, weak lin-17 expression was detected in the P10.p cell and both of its daughter cells. Authors failed to detect lin-17 expression in P7.p, Z1, Z4 and T cells. |
Expressed on cell membrane. |
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Expr1516
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About 8 hour after hatching, P cells descend into the ventral nerve cord and interdigitate to form a single row of cells of P1-P12. In both sexes, mab-5 was detected in P7-P10. In older larva, mab-5 was detected in P7-P12, but not more anterior cells. In hermaphrodites, expression pattern was similar to males with two exceptions. The p(9-11).app cells and p(9-11).p cells no longer express MAB-5 by L2. In male, descendants of P10 consistently express high levels of MAB-5, those of P9 and P11 express low levels, and those of P7 and P8 express barely detectable levels. P12 descendants express MAB-5 only until the division of P12.a. The descendants of P cells express comparable levels of MAB-5 with exceptions to Pn.p cells, they express lower levels than their neighbors. Expression levels in P descendants were similar to those seen in the neighboring juvenile motoneurons. Both sets of cells expressed MAB-5 in a graded pattern, tapering off toward the anterior. In newly hatched larva, in both sexes, mab-5 was detected consistently in nuclei of P9/10 and P11/12, but not more anterior P cells. mab-5 was also detected at high levels in the posterior juvenile motoneurons, located ventrally. MAB-5 is localized to the posterior of the body, including P7-P12 in the P lineage but not in more anterior cells. Within P7-P12, MAB-5 expression was graded with the highest levels in the P10 descendants. The descendants of any one P cell expressed comparable levels of MAB-5. |
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Expr12037
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During the early L3 stage, lin-17::GFP was expressed predominantly in P11.p and was barely detectable in P10.p. No expression was detected in P9.p. Subsequently, descendants of both P10.p and P11.p expressed lin- 17::GFP, with slightly higher levels in the P11.p descendants. |
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Expr12040
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BAR-1-GFP expression first appeared in P11.p in the late L1 stage. In the early-to-middle L2 stage, BAR-1-GFP accumulated in the cytoplasm of P11.p in a punctate pattern, presumably resulting from the stabilization of BAR-1 in response to increased Wnt signaling. The punctate GFP fluorescence in the cytoplasm of P11. p rapidly decreased during the mid-to-late L2 stage. By the mid-L3 stage, just before P11.p divides, BAR-1-GFP expression appeared to be brighter in the nucleus than in the cytoplasm. The switch of cytoplasmic-to-nuclear BAR-1-GFP accumulation is initiated in the mid-to-late L2 stage, coincident with the time window critical for the specification of HCG cell fates. BAR-1-GFP expression was undetectable in P10.p prior to cell division but became visible in the nucleus of the posterior daughter, P10.pp, suggesting that Wnt signaling through BAR-1 likely acts during fate execution of some descendants of the P10.p lineage. Although we did not observe lin-17::GFP expression in P9.p, faint, mostly cytoplasmic expression of BAR-1-GFP was sometimes seen in P9.p up to the mid-L2 stage, just before P9.p fuses with hyp7. |
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