WormMine: RNAi WBRNAi00107820

• WormBase ID: WBRNAi00107820
• Phenotype Remark: Figure 3, unsealed phagosomes can be labeled by PLCδ1-PH and the membrane impermeable dye FM4-64. Loss of mtm-1, piki-1, lst-4, dyn-1 or ocrl-1 shows phagosome sealing defective. "Inactivation of OCRL-1 causes persistent cell corpses, which are labeled by FM4-64, Annexin V, and PLCδ1-PH, indicating that PtdIns(4,5)P2 hydrolysis is required for sealing of apoptotic cell-containing phagosomes (Fig 2, P-Q'; Fig 3, G-G'', L, and M; and Fig S3, E-E''', H, and I). Consistent with the sealing defect, ocrl-1(lf) phagosomes were not acidified, and the recruitment of maturation factors (RAB-5 and RAB-7) and lysosomes (NUC-1) was blocked (Fig 2, R-U' and X)."
• Remark: ocrl-1 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007620	ocrl-1	C16C2.3	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C16C2.3b	C16C2.3b	816	I: 9715633-9715757
CDS:C16C2.3a	C16C2.3a	2139	I: 9715633-9715757

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0002394	phagosome sealing defective	The closure of a phagosome does not occur and it remains connected with the plasma membrane, leaving the lumen open to the outside. Complete closure usually occurs once an apoptotic cell has been surrounded by an engulfing cell.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00048406

0 Strain