WormMine: RNAi WBRNAi00088422

• WormBase ID: WBRNAi00088422
• Phenotype Remark: For SPL dsRNA-injected worms, all eggs laid after ~12 h post-injection demonstrated abnormalities, although these ranged broadly in severity from worms that did not develop beyond L1 or L2 stages to worms that developed to adulthood and demonstrated defects of adult structures. Compared with control F1s, animals inheriting spl-1 dsRNA developed slowly, moved sluggishly, and were thin, pale, and starved in appearance. The adults were smaller than controls and did not pump food actively. The treated animals reached adulthood 24-48 h later than controls. Adult hermaphrodites that inherited spl-1 dsRNA were markedly different from controls especially in the gonad and uterus. Although control animals had abundant nuclei in the distal gonad and a row of developing oocytes in the proximal gonad, affected hermaphrodites had poorly developed distal gonads with fewer nuclei. Control adults had embryos of progressive stages of development in the uterus, whereas the number of developing oocytes in the proximal gonad of affected hermaphrodites was significantly reduced. The embryos in the uterus of affected progeny were also morphologically abnormal. Those near the vulva were at late developmental stages, indicating a defect in egg laying. There was not a uniform progression of developmental stages in adjacent embryos suggesting a defect in ovulation or cell division. Embryonic and larval semi-lethality was observed, and some premature death of adults was also noted. None of these effects were found in worms that inherited B0222.4 dsRNA, which were similar in growth, appearance, and reproduction to uninjected worms. Furthermore, no synergistic effects were observed when B0222.4 and spl-1 dsRNA were injected together.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004981	spl-1	Y66H1B.4	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y66H1B.4	Y66H1B.4	1659	IV: 380018-380249

1 Laboratories

Primary Identifier
PS

8 Phenotype

Identifier	Name	Description
WBPhenotype:0000691	gonad development variant	Variations in the progression of the organ that produces gametes over time, from its formation to the mature structure compared to control.
WBPhenotype:0000006	egg laying defective	Eggs are not laid, laid at a slower rate or eggs are laid at a later stage of development compared to control.
WBPhenotype:0000062	lethal	Animals die prematurely at any stage of the life cycle.
WBPhenotype:0000848	developmental delay	The developmental progression of an animal over time, from its formation to its mature structure is delayed compared to control.
WBPhenotype:0000019	pharyngeal pumping reduced	Pharyngeal pumping motion occurs at a slower rate than control animals.
WBPhenotype:0000520	morphology phenotype	Animals exhibit variations in the form, structure or composition of any of its parts compared to control animals.
WBPhenotype:0000749	embryonic development variant	Variations in the progression of an embryo over time, from zygote formation to hatching compared to control.
WBPhenotype:0001944	oocyte number decreased	A decreased number of celullarized haploid female germ cells (oocytes) are present in the gonad compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005941

0 Strain