Experiment was done with limited exposure to RNAi, defined as the following: L4 hermaphrodites were placed on the Petri dish. These hermaphrodites were transferred to a new Petri dish daily, and progeny were scored for the Muv phenotype. We defined limited and extensive exposure to RNAi as progeny laid on day 1 and day 2 plates or day 3 and day 4 plates, respectively.
Multiple vulva-like protrusions are present along the ventral side of the animal. In C. elegans, this is usually a result of all six vulval precursor cells adopting primary or secondary vulval fates.
