WormMine: RNAi WBRNAi00087587

• WormBase ID: WBRNAi00087587
• Genotype: itIs153[pie-1::GFP::PAR-2]
• Phenotype Remark: Class I. Embryos in this class do not undergo ruffling, pseudocleavage or cytokinesis. Pronuclei form and migrate normally in these embryos, eventually resulting in the formation of a symmetric spindle. GFP:PAR-2 did not become visibly enriched on the cortex (n=3), and instead was maintained throughout the cytoplasm and also on foci around centrosomes. Class II and Class III. These embryos developed cytokinesis furrows that were either transient (Class II) or lead to the formation of two equal size cells (Class III). Unlike Class I embryos, however, Class II and Class III embryos developed one or two patches of GFP:PAR-2 at the cortex by pronuclear meeting (n=6). In all nmy-2(RNAi) embryos (n=8), GFP:PAR-2 was also detected in foci around the pronuclei or spindle. In four out of eight time-lapse analyses, GFP:PAR-2 appeared to shuttle from the cortex to the nearest pronucleus or centrosome.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003777	nmy-2	F20G4.3	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F20G4.3	F20G4.3	6012	I: 7927071-7927198

1 Laboratories

Primary Identifier
JH

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000961	pattern of transgene expression variant	Any variation in the cellular or subcellular pattern of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005735

0 Strain