WormMine: RNAi WBRNAi00092397

• WormBase ID: WBRNAi00092397
• Genotype: asd-2(yb1540); unc-60(su158)
• Phenotype Remark: asd-2 RNAi in conjunction with the asd-2(yb1540) mutation restored sinusoidal locomotion to the unc-60(su158) mutant and greatly improved the actin filament organization in body wall muscles, as determined by tetramethylrhodamine-phalloidin staining. asd-2 RNAi/yb1540 also resulted in ectopic expression of endogenous UNC-60A in body wall muscles in the unc-60(su158) mutant, as determined by UNC-60A antibody staining.
• Remark: (Figure 5F, S3D) asd-2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006423	asd-2	T21G5.5	Caenorhabditis elegans

4 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:T21G5.5a	T21G5.5a	987	I: 6879770-6880041
CDS:T21G5.5b	T21G5.5b	1212	I: 6878976-6879204
CDS:T21G5.5c	T21G5.5c	1338	I: 6878976-6879204
CDS:T21G5.5d	T21G5.5d	1461	I: 6877740-6877801

1 Laboratories

Primary Identifier
KH

3 Phenotype

Identifier	Name	Description
WBPhenotype:0004018	sinusoidal movement variant	Any variation in the propagation of the contraction sine wave along the body while an animal travels from one location to another, compared to control.
WBPhenotype:0001570	body wall muscle actin organization defective	The characteristic organization of actin filaments into I-bands is disrupted. In C. elegans, actin filaments no longer appear as thin continuous longitudinal stripes along the muscle quadrants.
WBPhenotype:0001404	ectopic expression antibody	Any variation that results in the detection of protein expression in cells/tissues through antibody staining, that is otherwise not observed in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00041641

0 Strain