WormMine: RNAi WBRNAi00077791

• WormBase ID: WBRNAi00077791
• Genotype: fem-3(q20)
• Phenotype Remark: rpn-10;ufd-2 (RNAi) overcomes the masculinizing effect of fem-3 (gf) q20. Oocyte production was restored in more than 70 percent of fem-3 (gf) mutant worms by treatment with rpn-10;ufd-2 (RNAi).
• Remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. Authors amplified by PCR from a C. elegans mixed stage cDNA library. Primer sequences are available from the author on request. fem-3(q20) is a gain of function allele. fem-3 (gf) alleles masculinize only hermaphrodite germlines.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004466	rpn-10	B0205.3	Caenorhabditis elegans
WBGene00006734	ufd-2	T05H10.5	Caenorhabditis elegans

4 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:B0205.3	B0205.3	1041	I: 10730928-10731299
CDS:T05H10.5a	T05H10.5a	2943	II: 8062752-8062808
CDS:T05H10.5b	T05H10.5b	2955	II: 8062752-8062808
CDS:T05H10.5c	T05H10.5c	2940	II: 8062752-8062808

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0001572	masculinized	Animals that are not ordinarily male, exhibit male-specific attributes. In C. elegans, XX animals exhibit male-specific attributes.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00028730

0 Strain