WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00091022 Genotype  unc-119(ed3) III; cbgIs91[pPept-1:pept-1::DsRed;unc-119(+)]; cbgIs98[pPept-1:GFP::rab-11.1;unc-119(+)]
Phenotype Remark  PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes Remark  (Table S1, S3, Figure 2a, 3f) M117.2/par-5 RNAi
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

2 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003920 par-5 M117.2 Caenorhabditis elegans
WBGene00010919 M117.3 M117.3 Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:M117.2a M117.2a 747   IV: 11820991-11821284
CDS:M117.2b M117.2b 381   IV: 11820991-11821284
CDS:M117.3 M117.3 378   IV: 11823516-11823602

1 Laboratories

Primary Identifier
MZE

5 Phenotype

Identifier Name Description
WBPhenotype:0001278 transgene expression reduced Any decrease in the intensity of expression of a gene product produced by exogenous DNA that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0000679 transgene subcellular localization variant Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0002094 RAB-11 recycling endosome morphology variant Any variation in the form or composition of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane.
WBPhenotype:0002095 lysosome-related organelle morphology variant Any variation in the form or composition of membrane bound organelles, known as lysosome-related organelles (LROs) that, like lysosomes, have an acidic interior and generally act to digest components of the cell but are distinct in function, composition, and morphology, compared to control.
WBPhenotype:0002107 RAB-11 recycling endosome localization variant Any variation in the subcellular localization of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00041129

0 Strain