WormMine: RNAi WBRNAi00077721

• WormBase ID: WBRNAi00077721
• Genotype: scrm-4(tm624) scrm-2(tm650); scrm-3(tm631)
• Phenotype Remark: tat-1 RNAi alters phosphatidylserine (PS) distribution in the membrane of C. elegans germ cells so that PS staining was observed on the surface of many normal germ cells. In wild-type C. elegans, no PS staining was observed on the surface of normal germ cells. Similar amounts of germ cell PS staining were observed in scrm-4(tm624) scrm-2(tm650); scrm-3(tm631) triple mutant animals treated with tat-1(RNAi) to that seen in wild-type animals treated with tat-1(RNAi). Thus, loss of phospholipid scramblases does not affect PS externalization in tat-1 deficient animals.
• Remark: Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. Authors state that the tat-1 RNAi construct was made by polymerase chain reaction (PCR) amplification of 310 bp of the tat-1 coding region, but dont specify which portion.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00013034	tat-1	Y49E10.11	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y49E10.11a	Y49E10.11a	3420	III: 12399363-12399460
CDS:Y49E10.11b	Y49E10.11b	3270	III: 12404132-12404204
CDS:Y49E10.11c	Y49E10.11c	3579	III: 12401682-12402063

1 Laboratories

Primary Identifier
CU

1 Phenotype

Identifier	Name	Description
WBPhenotype:0002178	plasma membrane leaflet composition variant	Any variation in the components that make up either of the two leaflets of the semipermeable bilayer surrounding a cell that separates the cell from its external environment compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00031830

0 Strain