WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00003044 Gene Name  lir-1
Sequence Name  ? F18A1.3 Brief Description  lir-1 encodes a LIN-26-like zinc-finger protein that shares a unique C2H2 motif together with LIR-2, LIR-3, and LIN-26; lir-1 is part of two distinct operons: it is the downstream gene in an operon with lir-2 and the upstream gene in a second, overlapping operon with lin-26; the precise role of lir-1 in C. elegans development and/or behavior is not yet known as neither loss nor gain of lir-1 function appears to result in clear abnormalities and RNAi experiments targeting lir-1 result in embryonic and larval lethality due to loss of lin-26 expression; lir-1 transcripts appear ubiquitous in early embryos, disappear, and then re-appear in later embryonic hypodermal and support cells; during larval development, a lir-1::lacZ fusion that should reflect expression of all transcripts is seen in nearly all cells with the exception of germ cells and post-L1 intestinal cells.
Organism  Caenorhabditis elegans Automated Description  Predicted to enable metal ion binding activity. Expressed in several structures, including head; intestinal cell; muscle cell; pharynx; and tail.
Biotype  SO:0001217 Genetic Position  II :0.5091±
Length (nt)  ? 10359
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00003044

Genomics

15 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:F18A1.3b.1 F18A1.3b.1 1127   II: 7672005-7682360
Transcript:F18A1.3c.1 F18A1.3c.1 1252   II: 7672008-7682360
Transcript:F18A1.3i.1 F18A1.3i.1 1149   II: 7672008-7682360
Transcript:F18A1.3l.1 F18A1.3l.1 1115   II: 7672008-7682360
Transcript:F18A1.3a.1 F18A1.3a.1 1200   II: 7672010-7682359
Transcript:F18A1.3d.1 F18A1.3d.1 1155   II: 7672010-7682359
Transcript:F18A1.3j.1 F18A1.3j.1 1052   II: 7681045-7682358
Transcript:F18A1.3m.1 F18A1.3m.1 1021   II: 7681045-7682361
Transcript:F18A1.3h.1 F18A1.3h.1 1032   II: 7681045-7682363
Transcript:F18A1.3f.1 F18A1.3f.1 1101   II: 7681051-7682359
Transcript:F18A1.3e.1 F18A1.3e.1 750   II: 7681165-7682201
Transcript:F18A1.3h.2 F18A1.3h.2 771   II: 7681462-7682360
Transcript:F18A1.3g.1 F18A1.3g.1 426   II: 7681609-7682083
Transcript:F18A1.3k.1 F18A1.3k.1 429   II: 7681609-7682140
Transcript:F18A1.3n.1 F18A1.3n.1 456   II: 7681609-7682201
 

Other

14 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:F18A1.3a F18A1.3a 891   II: 7672043-7672108
CDS:F18A1.3d F18A1.3d 903   II: 7672043-7672108
CDS:F18A1.3l F18A1.3l 921   II: 7672043-7672108
CDS:F18A1.3f F18A1.3f 711   II: 7681165-7681326
CDS:F18A1.3g F18A1.3g 426   II: 7681609-7681895
CDS:F18A1.3k F18A1.3k 429   II: 7681609-7681895
CDS:F18A1.3b F18A1.3b 930   II: 7672043-7672108
CDS:F18A1.3c F18A1.3c 756   II: 7672043-7672108
CDS:F18A1.3e F18A1.3e 750   II: 7681165-7681326
CDS:F18A1.3h F18A1.3h 465   II: 7681609-7681895
CDS:F18A1.3i F18A1.3i 894   II: 7672043-7672108
CDS:F18A1.3j F18A1.3j 714   II: 7681165-7681326
CDS:F18A1.3m F18A1.3m 741   II: 7681165-7681326
CDS:F18A1.3n F18A1.3n 456   II: 7681609-7681895

26 RNAi Result

WormBase ID
WBRNAi00116181
WBRNAi00116183
WBRNAi00044907
WBRNAi00044908
WBRNAi00008657
WBRNAi00066784
WBRNAi00064890
WBRNAi00068456
WBRNAi00068457
WBRNAi00068458
WBRNAi00068459
WBRNAi00068460
WBRNAi00030282
WBRNAi00024306
WBRNAi00116178
WBRNAi00116185
WBRNAi00116187
WBRNAi00116189
WBRNAi00064775
WBRNAi00068908
WBRNAi00025131
WBRNAi00073031
WBRNAi00073033
WBRNAi00073032
WBRNAi00064723
WBRNAi00092023

153 Allele

Public Name
gk963801
gk963053
gk962682
WBVar01241663
WBVar02079553
gk147380
WBVar01695614
WBVar01695613
WBVar01695612
WBVar01695611
WBVar02068153
gk677280
gk868393
WBVar02069265
WBVar01241665
gk743954
WBVar01604186
WBVar01438533
WBVar01438531
gk696117
WBVar01375723
WBVar01375700
h7558
h15851
h16890
gk438810
gk771850
gk634188
gk412020
gk748780

1 Chromosome

WormBase ID Organism Length (nt)
II Caenorhabditis elegans 15279421  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00003044 7672005 7682363 1

3 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  

0 Downstream Intergenic Region

93 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Transcripts of coding genes that showed significantly decreased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_depleted_coding-RNA
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Bacteria: E.faecalis strain OG1RF Transcripts that showed significantly increased expression after infection by E. faecalis OG1RF. Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. WBPaper00059754:E.faecalis_OG1RF_upregulated
  Transcripts that showed significantly higher expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). DESeq2, fold change >= 2, FDR <= 0.01. WBPaper00056826:SGP_biased
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:GABAergic-neuron_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:seam_expressed
  Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva daf-16(mu86);glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_downregulated_daf-16(mu86);glp-1(e2141)
  Transcripts that showed significantly decreased expression in day 3 adult hermaphrodite comparing to in L4 larva glp-1(e2141) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_downregulated_glp-1(e2141)
Bacteria diet: Sphingomonas aquatilis Yellow. Fed for 30 generations. Transcripts that showed significantly decreased expression after fed by bacteria Sphingomonas aquatilis (Yellow) for 30 generations comparing to animals fed by E. coli OP50. DESeq2 fold change > 2, p-value < 0.01. WBPaper00061007:S.aquatilis_downregulated
  Maternal class (M): genes that are called present in at least one of the three PC6 replicates. A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. [cgc5767]:expression_class_M
  Strictly maternal class (SM): genes that are the subset of maternal genes that are not also classified as embryonic. A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. [cgc5767]:expression_class_SM
  Transcripts that showed significantly increased expression in animals exposed to 400uM tamoxifen from L1 to L4 larva stage. DEseq2, fold change > 2 WBPaper00064505:tamoxifen_upregulated
  Transcripts that showed significantly changed expression in 6-day post-L4 adult hermaphrodite comparing to in 1-day post L4 adult hermaphrodite animals. Sleuth WBPaper00051558:aging_regulated
  Top 300 transcripts enriched in excretory duct, excretory pore according to single cell RNAseq. Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. WBPaper00061340:Excretory_duct_and_pore
  Transcripts enriched in AMso according to single cell RNAseq. Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched. WBPaper00061651:AMso_enriched
  Transcripts enriched in PHso according to single cell RNAseq. Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched. WBPaper00061651:PHso_enriched
  Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:Day10_vs_Day1_upregulated
Temprature shift to 28C for 24 hours. Transcripts that showed significantly decreased expression after animals were exposed to 28C temperature for 24 hours. Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. WBPaper00061341:28C_24h_downregulated
  Transcripts that showed significantly decreased expression in tetraploid N2 comparing to diploid N2 animals at L4 larva stage. DESeq2 R package (1.20.0), fold change > 2, and FDR < 0.05. WBPaper00066110:tetraploid_vs_diploid_downregulated
  Transcripts that showed significantly increased expression in isp-1(qm150) comparing to in N2. Differential gene expression analysis was performed using the quasi-likeli-hood framework in edgeR package v. 3.20.1 in R v. 3.4.1. WBPaper00053810:isp-1(qm150)_upregulated
  Transcripts detected in germline isolated from day-1 adult hermaphrodite animals. All three experiments have CPM >= 1. WBPaper00067147:germline_expressed
  Transcripts that showed decreased expression in hlh-11(ko1) knockout strain comparing to in wild type background. DESeq2, FDR < 0.05 WBPaper00060683:hlh-11(ko1)_downregulated
  Genes found to be regulated by low-copy overexpression of sir-2.1 with p < 0.014. N.A. WBPaper00026929:sir-2.1_overexpression_regulated
  TGF- Dauer pathway adult transcriptional targets. Results obtained by comparing the microarray results of the dauer-constitutive mutants daf-7(e1372), daf-7(m62), and daf-1(m40) with dauer-defective mutants daf-3(mgDf90), daf-5(e1386), and daf-7(e1372);daf-3(mgDf90) double mutants at the permissive temperature, 20C, on the first day of adulthood. SAM WBPaper00031040:TGF-beta_adult_downregulated
  Genes expressed in N2. Expressed transcripts were identified on the basis of a Present call in 3 out of 4 N2 experiments as determined by Affymetrix MAS 5.0. WBPaper00025141:N2_Expressed_Genes
  Transcripts with significantly increased expression in isp-1(qm150) vs. N2, and in isp-1(qm150) ced-4(n1162) vs. ced-4(n1162). Comparisons of each genotype were compared to the wild-type using the Empirical Base (Wright & Simon) algorithm and fold changes were represented on a log2 scale. A threshold of p < 0.05 and a fold change of 1.3 (log2) was set to determine differentially expressed targets. WBPaper00045263:isp-1(qm150)_upregulated
  Transcripts with significantly increased expression in nuo-6(qm200) vs. N2, and in nuo-6(qm200);ced-4(n1162) vs. ced-4(n1162). Comparisons of each genotype were compared to the wild-type using the Empirical Base (Wright & Simon) algorithm and fold changes were represented on a log2 scale. A threshold of p < 0.05 and a fold change of 1.3 (log2) was set to determine differentially expressed targets. WBPaper00045263:nuo-6(qm200)_upregulated

7 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1031428 Tiling arrays expression graphs  
Reporter gene fusion type not specified.   Expr1327 Expression of the lir-1::lacZ construct pML169, which should reflect the expression of all lir-1 isoforms, and of the lir-1::lacZ construct pML165, which should reflect only the expression of lir-1 isoforms starting after the second exon, were first very weakly detected at the 200-cell stage and then clearly detected at the 350-cell stage in major hypodermal cells and at the early comma stage in those same hypodermal cells, as well as in support cells and in all minor hypodermal cells. Starting at the late comma stage, expression of the lir-1::lacZ construct pML169, but not that of pML165, was detected in all cells (i.e., in intestinal, pharyngeal, muscle, neuronal, hypodermal, and support cells). Similarly, during larval development, expression of the lir-1::lacZ pML169 was detected in virtually all cells, including cells of the somatic gonad, but not in intestinal cells after the late L1 stage nor in germ cells.  
    Expr1324 lir-1 transcripts were detected by in situ hybridization in all cells between the 2- and 50-cell stages, disappearing after that stage. They reappeared between the 200- and 350-cell stages, but they were too weak to allow a clear identification of cells. At the comma stage, a signal was detected mostly in the anterior and ventral parts of the head and in the tail.  
    Expr1020151 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr2013224 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr1148878 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2031455 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  

3 GO Annotation

Annotation Extension Qualifier
  enables
  enables
  enables

0 Homologues

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00003044 7672005 7682363 1

3 Ontology Annotations

Annotation Extension Qualifier
  enables
  enables
  enables

0 Regulates Expr Cluster

1 Sequence

Length
10359

1 Sequence Ontology Term

Identifier Name Description
gene  

2 Strains

WormBase ID
WBStrain00026572
WBStrain00051951

1 Upstream Intergenic Region

WormBase ID Name Sequence Name Length (nt) Chromosome Location Organism
intergenic_region_chrII_7671910..7672004   95 II: 7671910-7672004 Caenorhabditis elegans