WormMine: Gene WBGene00000032

• WormBase Gene ID: WBGene00000032
• Gene Name: abu-9
• Sequence Name: R09F10.2
• Brief Description: abu-9 encodes a transmembrane protein (identical to that predicted for the pqn-57 gene) with a predicted signal sequence, a glutamine/asparagine-rich domain and multiple cysteine-rich repeats (DUF139); abu-9 expression is induced by blockage of the unfolded-protein response in the endoplasmic reticulum, and ABU-9 may help protect the organism from damage by improperly folded nascent protein.
• Organism: Caenorhabditis elegans
• Automated Description: Involved in endoplasmic reticulum unfolded protein response.
• Biotype: SO:0001217
• Genetic Position: X :-0.161131±
• Length (nt): 1233

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00000032

Genomics

1 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:R09F10.2.1	R09F10.2.1	1137	X: 8322111-8323343

Other

1 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:R09F10.2	R09F10.2	1137	X: 8322111-8322161

3 RNAi Result

• WBRNAi00051635
• WBRNAi00051636
• WBRNAi00017663

10 Allele

• gk964260
• gk962707
• gk964193
• gk964194
• gk963896
• gk963732
• gk964126
• tm11434
• tm11437
• WBVar01885147

1 Chromosome

• WormBase ID: X
• Organism: Caenorhabditis elegans
• Length (nt): 17718942

1 Chromosome Location

• Feature . Primary Identifier: WBGene00000032
• Start: 8322111
• End: 8323343
• Strand: 1

3 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)

0 Downstream Intergenic Region

27 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression in daf-2(e1370) comparing to in N2.	Student's t-test, fold change > 2, p-value < 0.05.	WBPaper00055386:daf-2(e1370)_upregulated
20C vs 25C	Transcripts that showed differential expression in 20C vs 25C in mir-34(OverExpression) animals at adult stage.	N.A.	WBPaper00050488:20C_vs_25C_regulated_mir-34(OverExpression)_adult
Bacteria infection: Serratia marcescens	Genes with increased expression after 24 hours of infection by S.marcescens Fold changes shown are pathogen vs OP50.	For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated.	WBPaper00038438:S.marcescens_24hr_upregulated_TilingArray
	Transcripts that showed significantly increased expression in jmjd-3.1p::jmjd-3.1 comparing to in N2.	DESeq2 Benjamini-Hochberg adjusted p-value < 0.05.	WBPaper00049545:jmjd-3.1(+)_upregulated
	Transcripts that showed significantly increased expression in rgef-1p::jmjd-1.2 comparing to in N2.	DESeq2 Benjamini-Hochberg adjusted p-value < 0.05.	WBPaper00049545:rgef-1p-jmjd-1.2(+)_upregulated
20C vs 25C	Transcripts that showed differential expression in 20C vs 25C in mir-34(gk437) animals at adult stage.	N.A.	WBPaper00050488:20C_vs_25C_regulated_mir-34(gk437)_adult
20C vs 25C	Transcripts that showed differential expression in 20C vs 25C in N2 animals at adult stage.	N.A.	WBPaper00050488:20C_vs_25C_regulated_N2_adult
	Transcripts that showed differential expression in dauer mir-34(gk437) vs dauer mir-34(OverExpression) animals at 20C.	N.A.	WBPaper00050488:mir-34(gk437)_vs_mir-34(OverExpression)_regulated_dauer_20C
	Transcripts that showed differential expression in dauer N2 vs dauer mir-34(gk437) animals at 20C.	N.A.	WBPaper00050488:N2_vs_mir-34(gk437)_regulated_dauer_20C
	Transcripts that showed significantly decreased expression in ash-2(RNAi) comparing to in wild type animals injected with vector.	Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.	WBPaper00066232:ash-2(RNAi)_downregulated_WT
	Transcripts that showed significantly increased expression in eat-2(ad465) comparing to in N2.	Student's t-test, fold change > 2, p-value < 0.05.	WBPaper00055386:eat-2(ad465)_upregulated
	Genes that showed significantly decreased expression after exposure to 1mg/L MWCNTs from L1 larva to young adult.	Transcripts with false discovery rate-corrected p-values < 0.05 and fold change > 2 were defined as differentially expressed.	WBPaper00049377:MWCNT_downregulated
	Transcripts that showed significantly decreased expression in animals fed with JM103 bacteria producing Cry5B, comparing to control animals fed with JM103.	ANOVA, p-value < 0.05.	WBPaper00056167:Cry5B_downregulated
	Transcripts that showed significantly decreased expression in lin-22(ot269) comparing to in N2 at L3 larva.	Differences in gene expression were then calculated using the negative binomial test in the DESeq package (FDR = 0.1).	WBPaper00053295:lin-22(ot269)_downregulated
	Genes that were upregulated during lethargus (sleep-like) stage with oscillating expression, according to microarray analysis comparing L3 lethargus to L4 large, and L4 lethargus to young adult.	Genes were counted as significantly up or down regulated for L3 sleep-like if the calculated false discovery rate (FDR) was equal or smaller then 5.0% and the fold change was > 2. p-values were obtained from the moderated t-statistic and corrected with the Benjamini-Hochberg method.Only genes consistently up or down regulated in both the L3 and L4 molt were selected to eliminate the effect of developmental progression. Authors reasoned that gene expression changes that would be biologically relevant would oscillate with substantial amplitude in phase with the molting cycle. They applied an arbitrary threshold and selected only those genes that oscillated with an amplitude of at least 50%.	WBPaper00046024:sleep_upregulated_oscillating
	Coexpression clique No. 298, pqn-54-abu-9, on the genome-wide coexpression clique map for the nematode GPL200 platform.	All available microarray datasets for the GPL200 platform (Affymetrix C. elegans Genome Array) were obtained from the GEO repository. This included 2243 individual microarray experiments. These were normalized against each other with the software RMAexpress (Bolstad, 2014). Based on these normalized values, Pearsons correlation coefficients were obtained for each probe-probe pair of the 22,620 probes represented on this array type. The resulting list of correlation coefficients was then ranked to generate the ranked coexpression database with information on each probe represented on the GPL200 platform.	WBPaper00061527:pqn-54-abu-9
	Transcripts that showed significantly decreased expression in adr-1(tm668) and adr-1(gv6) comparing to in N2 at L4 larva stage.	DESeq FDR <= 0.05	WBPaper00056617:adr-1_downregulated_L4_transcript
moderate static magnetic field	Transcripts that showed significantly increased expression after exposure to moderate static magnetic field.	N.A.	WBPaper00064509:static-magnetic-field_upregulated
	Genes with at least 2-fold greater expression in par-1 emrbyos (with excess pharyngeal cells) comparing to skn-1 embryos (with no pharynx) during microarray analysis.	Genes with at least 2 fold change in expression are considered differentially expressed.	WBPaper00024505:pharyngeal_enriched
	Transcripts that showed significantly increased expression in csr-1a(tor159) comparing to in N2 at 20C.	DESeq2, fold change > 2, p-value < 0.05.	WBPaper00061753:csr-1(tor159)_upregulated_20C
	Transcripts that showed significantly increased expression in hsf-1(RNAi) comparing to in wild type animals injected with vector.	Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.	WBPaper00066232:hsf-1(RNAi)_upregulated_WT
	Genes that are significantly down regulated in tdp-1(ok803) poly(A) RNA-seq verses in N2.	DESeq v1.14, with cut-off p-value < 0.05 and FDR < 0.1.	WBPaper00046012:tdp-1(ok803)_downregulated
	Transcripts that showed significantly increased expression in hsf-1(RNAi) comparing to in ash-2 animals injected with vector.	Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.	WBPaper00066232:hsf-1(RNAi)_upregulated_ash-2
	Genes that are upregulated by 0.2M ethanol treatment.	Differences in gene expression levels between the two groups of worms were analyzed with Cufflinks 1.0.3, using a cutoff of twofold difference and FDR corrected p < 0.05.	WBPaper00041954:ethanol_upregulated
	Genes that showed significantly increased expression in animals fed with E. coli strain expressing alpha-neurotoxin rBmTX14, comparing to in control animals.	MAS 2.0	WBPaper00050109:rBmTX14_upregulated
	Transcripts that showed significantly decreased expression in adr-2(ok735) and adr-2(gv42) comparing to in N2 at L4 larva stage.	DESeq FDR <= 0.05	WBPaper00056617:adr-2_downregulated_L4_transcript
	Genes whose activation by tunicamycin treatment is enhanced in xbp-1(zc12)III mutant animals.	A t-test was used to determine the probability that differences in treatment-induced levels of mRNA between the wild type and xbp-1 mutant were due to chance. A 95% confidence limit was applied.	WBPaper00005432:xbp-1(zc12)_upregulated

3 Expression Patterns

• Primary Identifier: Expr1155307
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr2027349
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1011445
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

1 GO Annotation

0 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00000032
• Start: 8322111
• End: 8323343
• Strand: 1

1 Ontology Annotations

0 Regulates Expr Cluster

1 Sequence

• Length: 1233

1 Sequence Ontology Term