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Genome-wide analysis of developmental and sex-regulated gene expression profile. |
self-organizing map |
cgc4489_group_11
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Differentially-expressed genes in the MA lines. |
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WBPaper00025099:MA_specific
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Transcripts that showed significantly increased expression in elli-1(sam-3) comparing to in N2 animals, accordingto tiling array analysis. |
Fold change > 1.2, q-value < 0.05. |
WBPaper00050743:elli-1(sam-3)_upregulated
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Genes enriched in intestine. |
To identify genes that are significantly enriched by mRNA tagging, we first normalized the total amount of Cy3 and Cy5 signal to each other in each hybridization. We measured the ratio of the signals from the co-immunoprecipitated mRNA (Cy5) to total RNA in the cell extract (Cy3), and calculated the percentile rank for each gene relative to all genes in each hybridization. The mean percentile rank was determined from eight repeats of the mRNA-tagging experiment. Student's t-test was used to determine which genes showed a mean enrichment significantly greater than the median enrichment for all genes (P<0.001). |
WBPaper00026980:intestine_enriched
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Genes with more than 2 fold increase of expression after 500 mg/l acrylamide treatment comparing with control. |
Two-sided t-test, p < 0.005. |
WBPaper00031184:acrylamide_upregulated
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Germline-enriched and sex-biased expression profile cluster D. |
hierarchical clustering |
[cgc6390]:Cluster_D
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spermatogenesis-enriched |
Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. |
[cgc6390]:spermatogenesis-enriched
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Fungi infection: Fusarium oxysporum |
Transcripts that showed significantly altered expression after infected by fungi Fusarium oxysporum from 0 t0 48 hours. |
The Benjamini and Hochberg approach was used to adjust the resulting P-values for controlling the false-discovery rate. The transcripts having a false-discovery rate of < 0.05 were considered as differentially expressed. |
WBPaper00053368:F.oxysporum_regulated
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