WormMine: AnatomyTerm WBbt:0004457

• Definition: Pharyngeal neurosecretory motorneuron, contain serotonin
• Name: NSML
• Primary Identifier: WBbt:0004457
• Synonym: lineage name: ABaraapapaav

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABaraapapaav	ABaraapapaav nucleus		WBbt:0002439

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 116_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:116_0

122 Expression Patterns

• Primary Identifier: Expr4817
• Remark: Picture: Fig. 2G.
• Pattern: In wild-type animals, GFP was observed in all identified serotonergic and dopaminergic neurons and many muscle cells. Please note: this strain -JY739- shows expression in biogenic amine neurons and the epidermis, not in muscle cells (Loer et al., 2015. Genetics. WBPaper00046585, Expr12165).

• Primary Identifier: Expr4227
• Remark: Reporter gene fusion type not specified.
• Pattern: Expressed in NSM and ADF neurons.

• Primary Identifier: Expr12930
• Pattern: A GFP reporter under the pfk-1.1 promoter is expressed in all examined neurons, including the NSM neuron.
• Subcellular Localization: Under normoxic conditions PFK-1.1 is localized in a punctate pattern at some of the cell somas and largely diffuse throughout the neurites. Upon exposure to hypoxia, PFK-1.1 becomes clustered in neurites, preferentially localizing to synaptic-rich regions and within 0.2 mm from vesicle release sites.

• Primary Identifier: Expr15649

• Primary Identifier: Expr10053
• Pattern: The nhr-239::gfp reporter was weakly expressed in three to four pairs of neurons in the head and a pharyngeal neuron in late stage embryos and all larval and adult stages. One pair of dorsal neurons express nhr-239::gfp very consistently and appear to be sensory, as do one pair of pharyngeal cells that appear to be the MC, NSM or M3 neurons.Faint fluorescence was observed in the pharynx (which may be an artefact), but did not observe nhr-239 expression in other cells at any stage. As expected from the modest expression levels observed in qRT-PCR experiments, fluorescence from the nhr-239::gfp transgene was very faint. While it is possible that the translational fusion did not recapitulate the entire nhr-239 expression pattern, this result suggests that nhr-239 is expressed only in a very limited subset of neurons. nhr-239 transcripts were identified by real-time qRT-PCR at very low levels (no more than 2x10-6 the level of 18 S rRNA standards) and displayed a more complicated pattern, increasing somewhat from embryos to L1, before declining progressively during later larval development.

• Primary Identifier: Expr11451
• Pattern: mgl-3 is expressed throughout the life of the NSM neurons.

• Primary Identifier: Expr12338
• Pattern: In worms expressing mgl-3::GFP as an extra-chromosomal array GFP fluorescence was detected in the pharyngeal nervous system, specifically NSML/R and in the nerve ring.

• Primary Identifier: Expr8247
• Remark: Picture: Figure 5D.
• Pattern: Pmgl-3::gfp was expressed in NSM, ADF, ASE, and AWC amphid sensory neurons, and the RIB and RIC interneurons. Occasional expression in BAG-ciliated neurons was also noted.

• Primary Identifier: Expr15388

• Primary Identifier: Expr12168
• Pattern: qdpr-1 was expressed in the epidermis -similar to what observed in cat-4 transgenics- but was not highly expressed in 5HT and DA neurons. These transgenics all also showed some expression of varying intensity in other cells (non-epidermal cells, and non-5HT and non-DA neurons in the head and body). A qdpr-1 full-length translational fusion was expressed in several known 5HT and DA neurons, including NSMs, ADFs, CEPs, and other cells.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr822
• Remark: C43H6.9 = glr-7
• Pattern: I3, I2, I6, MI, NSM, I1(?).

• Primary Identifier: Expr15579

• Primary Identifier: Expr823
• Remark: F22A3.3 = glr-8
• Pattern: I1, I2, MC, NSM, M3 (left/right), I3, MI M4, M3 (left/right), I6, M5, BDU, ALM, PLM, URB (left/right)

• Primary Identifier: Expr15583

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr13158

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr11622
• Pattern: Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx.

• Primary Identifier: Expr8850
• Remark: Picture: Fig 3.
• Pattern: Neuronal Expression: AVA, AVB, AVE, PVC, AIB, AUA, AVG, RIB, RIC, SAA, SIA, SIB, RIF, RIM, RMD, RME, SMD, DA, DB, VA, VB, M5, NSM, MC, I3, MI?. Non-neuronal Expression: rectal epithelium, body wall muscle, spermethecae, vulva muscle.

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018

2 Parents

Definition	Name	Synonym	Primary Identifier
embryonic cell	ABaraapapaa		WBbt:0006455
Neuron class of two pharyngeal neurosecretory-motor neurons.	NSM	neurosecretory-motor neuron	WBbt:0003666