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Picture: Figure 4. |
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Expr4900
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UNC-69::GFP expression was first detectable in embryos. In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of unc-69 in these latter cells was confirmed using an unc-69::LacZ::NLS fusion. |
In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in the cell bodies of neurons. |
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Expr13713
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Expression of pghm-1::GFP, pgal- 1::CFP and pamn-1::mCherry fusion constructs showed co-localization of the three reporter constructs in most of the cell bodies of the nerve ring in the head and the tail ganglia. Expression data indicate that neuropeptide PHM, PAL and PAM enzymes are expressed throughout the nematode nervous system, where they likely function redundantly to promote neuropeptide synthesis. |
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Expr1200076
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Data from the TransgeneOme project |
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Expr9600
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paqr-2 is expressed in head ganglion neurons, head muscle cells, the two pharyngeal M2 neurons, gonad sheath cell, seam cells, some ventral nerve cord and tail neurons, and occasionally in body muscles and intestine. |
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Expr12085
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plr-1::GFP expression became visible in late gastrulation stage embryos. Strongest expression was detected in many cells in the tail region and by comma stage the most prominent expression was seen in body wall muscle cells. In early larval stages expression was detectable in a number of different tissues including the major hypodermal cells, muscle and marginal cells of the pharynx, the intestine (strongest in the anteriormost and posteriormost cells) as well as the anal depressor and stomatointestinal muscle. In the nervous system GFP expression was visible in a few neurons in head ganglia, many ventral cord motor neurons and several neurons in the tail ganglia including the PDA neuron. Based on the lack of commissures the motor neurons are likely of the VA, VB, VC and/or AS class. Based on the number of cells the majority (or even all) of these classes express GFP. In general expression was strongest in the tail region throughout development. This also held true for the motor neurons in the ventral cord, where GFP was strongest in the posterior-most cells and barely detectable in anterior motor neurons. Expression is maintained throughout larval development. In later larval stages expression is also seen occasionally in the distal tip cell of the developing gonad, the vulva and uterine muscle cells and the VC4 and VC5 neurons flanking the vulva. Expression in AVG, HSN or CAN neurons was not detectable with this reporter construct, but was detected in a previous study in HSN and CAN using a longer genomic construct (Moffat et al., 2014, Expr11480). |
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To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences. |
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Expr946
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GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr652
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beta-gal detected from late embryogenesis to adulthood. L1 expression restricted to gut cells (intestinal cells). L2-adult staining in gut, muscle, hypodermis, other epithelial cells, vulval cell and the nervous system (ganglia in the head and tail region and ventral nerve cord) |
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Expr2880
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Relatively strong expression in ganglia in the head and tail and in the anterior pharynx. GFP was not seen in ASI, ASJ, ADF or ASG (0 of 86 animals). Fluorescence in the anterior ganglion, where XXX is found, is at least an order of magnitude less than in the bright cells of the ganglia posterior to the nerve ring. The weakness of fluorescence has precluded identification of specific cells, but sometimes weak fluorescence was seen in the ventral, anterior part of the ganglion, where XXX resides (seven out of 53 animals). A small number of animals show weak expression in the hypodermis, muscles, intestine and distal tip cells. |
DAF-5::GFP from the rescuing construct mostly localizes to nuclei, which is consistent with the idea that DAF-5 is a transcription factor and functions in the nucleus. The intensity of fluorescence and nuclear localization of DAF-5::GFP from the rescuing construct was examined in wild-type and in TGF pathway mutants, including dauers, and no obvious differences was seen. |
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Expr2881
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The non-rescuing GFP construct is more strongly expressed, and shows more consistent expression in the hypodermis, muscles, intestine and distal tip cells; still, its expression is strongest in the head and tail ganglia. |
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Expr1200169
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Data from the TransgeneOme project |
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Expr11508
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the sta-1 promoter::NLS::GFP reporter gene was widely expressed in a variety of cells and tissues, during most life stages. Specifically, it was expressed at higher levels in pharynx compared to expression levels in other tissues. GFP was also apparent in the entire intestine, marking all nuclei. Fluorescence intensities of GFP appeared weaker in the anterior intestine than in the posterior intestine, but this effect was due largely to the anterior part of the intestine being obscured by the gonad, where no reporter gene expression was observed. GFP expression was also observed in body muscles as well as in most of the nervous system. Among neuronal cells, GFP expression was readily observed in head ganglia, particularly in the posterior ganglia, including the small dorsal ganglion, two lateral ganglia, and ventral ganglion. Similarly, the sta-1 promoter was functional in the two lumbar ganglia, the dorsorectal ganglion of the tail, and the ventral nerve cord. Developmentally, reporter gene expression was first observed in enclosure stage embryos in a variety of cells, and expression persisted throughout embryogenesis, the four larval stages, and the entire adult life span. Neither cell- nor tissue-specific expression pattern changes were observed during development, nor was there any evident modulation of expression level, as judged by fluorescence intensity. Furthermore, GFP expression persisted throughout the dauer larval stage, although GFP expression level decreased significantly, particularly in the pharynx. Similar conclusions concerning expression pattern were suported by analysis of protein distribution by immunofluorescence. |
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Expr1200005
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Data from the TransgeneOme project |
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Expr1200015
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Data from the TransgeneOme project |
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Expr1200043
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Data from the TransgeneOme project |
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Expr1200063
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Data from the TransgeneOme project |
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Expr1200071
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Data from the TransgeneOme project |
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Expr1200089
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Data from the TransgeneOme project |
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Expr1200176
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Data from the TransgeneOme project |
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Expr1200184
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Data from the TransgeneOme project |
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Expr1200190
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Data from the TransgeneOme project |
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Expr1200207
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Data from the TransgeneOme project |
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Picture: Figure 4D to 4F. |
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Expr8291
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Expressed in head neurons, spermatheca, gut, body wall muscles. |
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Picture: Figure 4G to 4H. |
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Expr8292
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Expressed in head neurons, spermatheca, gut, pharynx, vulva, phasmids. |
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Other strain-- UL403 late embryo(author) = elongating embryo + fully-elongated embryo(curator). |
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Expr122
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Expression begins in precomma stage embryos. It is quite strong, with extensive diffuse cytoplasmic staining as well as nuclear localised staining. Expression is strongest in young larvae, with staining observed in the ventral nerve cord, the circumpharyngeal nerve ring, the head ganglion, the tail ganglion, the retrovesicular ganglion, and in the developing vulva. In older larvae and in adults the strong pharyngeal expression seen in young larvae is less intense and some neuronal processes in the head become apparent (e.g. the motorneuron M1). There is also staining in the pharyngo-intestinal valve and in the seam cells, though expression appears to exclude the nuclei and is generally intermittent along the seam. The defecation muscle group stain as does its axon, DVB. The dorsal cord also stains but is very faint. Two commissures stain (these are also faint), one is located anterior to the vulva, and the other is posterior to the vulva. |
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GFP expression matched the expression pattern of a daf-4::gfp gene fusion (Patterson et al., 1997), suggesting that daf-4 regulatory sequences conferring tissue-specificity are upstream of the transcription start site. |
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Expr948
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GFP was expressed in the pharynx, intestine, hypodermis and body wall muscles, in L1 through adult stages. In the head, GFP was seen in neurons of the lateral, vesicular and retrovesicular ganglia. Ventral cord neurons also were visible, as was the PVT neuron, but only two phasmid neurons were detected in the tail. Whereas the nervous system is the primary site of gfp expression mediated by the daf-1 promoter, the daf-4 promoter directs expression more broadly, consistent with its other functions. In L1 larvae, when the dauer/nondauer decision is made, daf-4 promoter is active in neurons in the head, as well as in the ventral cord and tail. The promoters continues to express GFP in dauer larvae from starved plates. |
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Other strain-- UL1104 |
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Expr2069
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Extensive weak diffuse expression is seen in early and late stage embryos. In larvae and adults expression is still diffuse but more mosaic and is seen in a number of tissues, i.e. excretory cell, head ganglia, intestine, pharynx, and developing gonad. |
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Picture: Figure 4. |
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Expr7838
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UNC-69::GFP expression was first detectable in embryos. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of unc-69 in these latter cells was confirmed using an unc-69::LacZ::NLS fusion. Taken together, these results indicate that unc-69 is expressed widely, perhaps ubiquitously, in the C. elegans nervous system. |
In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. |
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life_stage summary : post L2 life_stage summary = post L1 or post L2 |
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Expr18
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Earliest expression is seen from L1 stage. It is seen in three neurons in the head ganglia. Nuclei of the lateral/ventral ganglia show expression from L2/3 onwards. Further staining of 3 cells, possibly in the tail ganglia, develops in adult worms along with a few nuclei in the ventral cord. Cytoplasmic staining of the neural processes in the ventral nerve cord occurs as well. nuclei of the lateral/ventral ganglia show expression from L2/3 onwards. Further staining of 3 cells, possibly in the tail ganglia, develops in adult worms along with a few nuclei in the ventral cord. |
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pre-hatched embryo(author) = fully-elongated embryo(curator). |
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Expr23
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About 6 nuclei in the major nerve ring are seen from hatching onwards. The number decreases to 4 by the onset of adulthood. Up to 8 neural nuclei of the head ganglia stain from the pre-hatched embryo stage to adulthood, a few of the cells also showing axonal process staining. Pre-hatched worms also harbour staining at the pharyngeal lumen. |
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Legacy Data: Author "Lynch AS" "Bauer PK" "Hope IA"Date 1996-06. |
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Expr64
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Embryonic stages reveal nuclear staining in as yet unidentified nuclei. These are likely to be neuroblasts however, as larval stages exhibit staining in the head ganglia and the ventral nerve cord. |
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