WormMine: ExpressionPattern Expr1626

• Pattern: GFP fluorescence was abundant in circumferential annular rings overlying muscle of 3 fold embryos. The antipeptide antibody raised to the intracellular domain showed similar staining. In contrast, this pattern was never observed in mutants; although mutant and wild-type (WT) embryos also exhibit diffuse patterns of antibody reactivity. MUP-4 continues to localize to circumferential rings over body wall muscle in larvae and adults. MUP-4:: GFP and MUP-4 antibodies also localize to other regions where cells show mechanical attachments to the hypodermis including the inner surface of the pharynx where the radial pharyngeal muscle cells attach to the marginal hypodermal cells, which secrete a cuticle; overlying anal and intestinal muscles; overlying vulval and uterine sex muscles; male tail muscle attachment zones; and the six mechanosensory neurons that mediate touch sensitivity through their attachment to the hypodermis. Examination of WT animals also labeled with mAb MH27, which recognizes adherens junctions, shows that MUP-4 staining can extend beyond the muscle to the seam cell boundary. In some mup-4::gfp-expressing lines, high concentrations of protein was often seen around the hypodermal or touch neuron nuclei. MUP-4 continues to localize to circumferential rings over body wall muscle in larvae and adults. MUP-4::GFP and MUP-4 antibodies also localize to other regions where cells show mechanical attachments to the hypodermis including the inner surface of the pharynx where the radial pharyngeal muscle cells attach to the marginal hypodermal cells, which secrete a cuticle; overlying anal and intestinal muscles; overlying vulval and uterine sex muscles; male tail muscle attachment zones; and the six mechanosensory neurons that mediate touch sensitivity through their attachment to the hypodermis. Examination of WT animals also labeled with mAb MH27, which recognizes adherens junctions, shows that MUP-4 staining can extend beyond the muscle to the seam cell boundary. In some mup-4::gfp-expressing lines, high concentrations of protein was often seen around the hypodermal or touch neuron nuclei.
• Primary Identifier: Expr1626
• Subcellular Localization: The observed pattern of MUP-4 is reminiscent of the pattern of the MH4 mAb, which recognizes IFs of hemidesmosomes. The relationship between MUP-4 and IF localization was further examined by overlay of confocal images of double-labeled animals. This analysis demonstrated that their patterns largely coincide. In addition to the annular localization, MUP-4 and IF proteins are sometimes seen diffusely throughout the hypodermal syncytial cell cytoplasm, possibly reflecting protein not yet recruited to the regions overlying muscle. MUP-4 also shows coincident localization with the MH4 IF in other structures such as the touch neuron channels, further supporting that MUP-4 associates with IFs.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neurons that sense body touch, have specialized microtubules in processes.	touch receptor neuron	microtubule cell	WBbt:0005237
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003497	mup-4	K07D8.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020