|
Moreover, neither the dgn-1::GFP promoter reporter nor the rescuing DGN-1::GFP fusion show expression in muscle. Plasmid pJJ516 was made by inserting GFP from pPD114.38 into the HindIII site near the end of the dgn-1 coding sequence. The product contains GFP inserted after residue 575 of DGN-1, with the final seven DGN-1 residues at the C terminus. Expression of DGN-1::GFP is identical to that of the dgn-1::GFP promoter reporter, and DGN-1::GFP rescues the sterility of dgn-1(cg121). |
|
Expr4218
|
In early (pre-morphological) embryos, dgn-1::GFP expression is evident in many epithelial and neural precursors comprising the outer layer of cells. As elongation begins at comma stage, expression becomes most prominent in several specialized epithelial cells, including pharyngeal e2 and marginal cells, excretory cells, the somatic gonad precursors (SGPs) Z1 and Z4, and rectal epithelial cells. Weaker expression is apparent in hypodermal precursors and neuroblasts along the ventral midline. Pharyngeal expression persists through the L3 larval stage, whereas excretory and rectal cell expression persists throughout development. SGP expression persists in SGP descendants, such as the distal tip cells (DTCs), and increases throughout the gonad during the L4 stage. Variable, generally weak expression is seen throughout larval development in several neurons, although PVP neurons show strong expression throughout development. Transient increased expression occurs in new P cell-derived neurons in the ventral nerve cord in late L1/early L2 stage animals. Variable weak expression is seen in hypodermal cells, principally hyp5 in the head. Preceding the L4/adult molt, expression increases in the vulval epithelium. |
|
|
|
|
Expr1200015
|
Data from the TransgeneOme project |
|
|
|
|
Expr1200036
|
Data from the TransgeneOme project |
|
|
|
|
Expr1200068
|
Data from the TransgeneOme project |
|
|
Reporter construct derived from clones that rescue emb-9 phenotype. Transgenic Marker: rol-6(su1006). late embryo(author) = fully-elongated embryo(curator). |
|
Expr518
|
Body wall muscle cells, hmc and MS.pppaaa show expression in early embryo. In later embryo through adulthood, stain accumulates between the hypodermis and four body muscle quadrants, and surrounds the pharynx, intestine and primordial gonad at L1 through adulthood. Presumptive GLRDL, GLRDR, GLRL, GLRR, GLRVL, GLRVR, coelomocytes, and anal muscles show expression in addition to body muscle cells at L1, L2, L3 L4 and adult. Distal tip cells of developing gonad in L2, L3, and L4. Spermathecal cells and distal ends of gonad show expression at L4 and adult. Vulval and uterine muscles in adult. Also expressed in two to four posterior intestinal cells. |
Preceding lima stage, intracellular. Later, extracellular around muscle quadrant-hypodermis interface, pharynx, intestine and gonad. Gonad and muscle stain intracellularly, pharynx and hypodermis do not. Four antibodies to type IV collagen gave same expression pattern. |
|
|
|
Expr1200103
|
Data from the TransgeneOme project |
|
|
|
|
Expr12123
|
WRM-1 was expressed in both seam cells and somatic gonadal precursors. |
|
|
|
|
Expr12184
|
Fluorescence microscopy of trd-1::gfp shows a strong expression pattern of trd-1 in the cytoplasm of seam cells. In addition, trd-1 expression was observed in the developing gonad, including strong expression in the DTC and in the spermatheca from the late L3 stage onwards. Additionally, expression of trd-1::gfp was observed in the Pn.p cells and anchor cell, as previously reported. |
|
|
|
|
Expr1200101
|
Data from the TransgeneOme project |
|
