WormMine: ExpressionPattern Expr15100

• Pattern: nekl-2 and nekl-3. Consistent with our previous study (YOCHEM et al. 2015), these reporters were expressed strongly within the epidermis beginning in late embryonic development. NEKL-2::NeonGreen was present in cytosolic puncta that were enriched at the hyp7-seam cell boundary, similar to our observations for MLT- 2 and MLT-4. As anticipated, NEKL- 2::NeonGreen showed extensive colocalization with MLT-2::mKate2 in the apical region of hyp7, although some puncta did not overlap. In the case of NEKL-2 and MLT-3, coexpression was more variable. In apical regions, including the region encompassing the hyp7-seam cell boundary, most NEKL-2::NeonGreen and MLT- 3::mKate2 puncta failed to overlap, although colocalization of some puncta was often observed. Moreover, in some animals we observed moderate-to-strong overlap, particularly in larger apical accumulations and in more medial planes.
• Primary Identifier: Expr15100

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022631	nekl-2	ZC581.1	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023