WormMine: ExpressionPattern Expr2695

• Pattern: Wild-type N2 and daf-2(e1370) animals carrying an extrachromosomal array that expressed GFP::LGG-1 had diffuse cytoplasmic GFP expression in multiple tissues in different stages of development, including the nervous system, pharynx, intestine, hypodermis, somatic gonad, and vulva. The tissue distribution of GFP::LGG-1 expression was similar to that observed for GFP regulated by the endogenous bec-1 promoter.
• Primary Identifier: Expr2695
• Subcellular Localization: In N2 animals, similar numbers of GFP-positive punctate areas were observed during growth at 15 centigrade and at 25 centigrade. In contrast, there was a marked increase in the number of seam cell, GFP-positive, punctate areas in daf-2(e1370) animals during dauer formation at 25 centigrade as compared to during reproductive growth at 15 centigrade (P < 0.001, t-test). In non-dauser stages, GFP::LGG-1 had diffuse cytoplasmic GFP expression. During dauer formation, a marked change was observed in the subcellular localization pattern of GFP::LGG-1 in hypodermal seam cells.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002980	lgg-1	C32D5.9	Caenorhabditis elegans

0 Life Stages