WormMine: ExpressionPattern Expr3470

• Pattern: The 3.1-kb promoter region of the sup-12 gene was able to drive expression of a reporter GFP in body wall muscle and pharynx.
• Primary Identifier: Expr3470
• Remark: Although the myo-3 promoter is active in the body wall muscle but not in the pharynx, myo-3 driven expression of GFP-SUP-12 was sufficient to rescue the Sup-12 mutant phenotype, indicating that GFP-SUP-12 is functional and expression of SUP-12 in the body wall muscle is functionally important for its interaction with unc-60. These results strongly suggest that SUP-12 is a muscle-specific regulator of pre-mRNA splicing in the nucleus.
• Subcellular Localization: GFP-tagged SUP-12, which was expressed in the body wall muscle under the control of the myo-3 promoter, predominantly localized to the nuclei. Within the nuclei, GFP-SUP-12 was found in diffuse and often speckled patterns in the nucleoplasm but excluded from the globular region, possibly representing the nucleolus. The speckled localization of SUP-12 in the nucleus is similar to patterns that are commonly observed for other splicing factors.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006321	sup-12	T22B2.4	Caenorhabditis elegans

0 Life Stages