WormMine: ExpressionPattern Expr8651

• Pattern: Immunostaining of wild-type worms using B74 or B78 revealed that endogenous ALP-1 proteins are highly expressed in the body wall muscle throughout postembryonic development. In addition, B78 (against ALP-1A, B, and D) also detects ALP-1 proteins at the apical and basal surfaces of the pharynx, another musculature structure in the worm, but at a very low level of expression.
• Primary Identifier: Expr8651
• Remark: Picture: Figure 2.
• Subcellular Localization: To determine in which sub-region ALP-1 proteins are located, we colabeled body wall muscle with antibodies directly against ALP-1(B78) and vinculin. Double labeling with anti-ALP-1 and anti-vinculin antibodies revealed both proteins are localized at dense bodies, but ALP-1 is absent from muscle cellcell junctions (dense plaques) that contain vinculin. High magnification views of the dense bodies revealed that ALP-1 proteins colocalize with anti-actinin, but only have limited overlap with vinculin. These data indicate ALP-1 and -actinin colocalize within the dense body in a region that is spatially distinct from the basal, membrane proximal zone that accumulates vinculin. Within body wall muscle, anti-ALP-1 staining appeared in a periodic punctate array that was identified as dense bodies by colabeling with an anti-actinin antibody. It should be noted that in body wall muscle, using anti-ALP-1 antibodies authors did not observe any nuclear staining, such as that reported for the ALP-1::GFP translational reporter.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001132	alp-1	T11B7.4	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024