WormMine: ExpressionPattern Expr8718

• Pattern: During larval development CeVPS-32-positive vesicles are still detected in epithelial tissues, such as the epidermis, the pharynx, the intestine, the vulva, the somatic gonad and the spermatheca. In addition, there was an accumulation of punctuate CeVPS-32 expression in the male germ line in both hermaphrodites and male adults. A careful analysis revealed that CeVPS-32 is not localized in germ cells but in residual bodies which are transient cytoplasts formed between the second meiotic division of secondary spermatocytes and formation of spermatids. During the rapid cell divisions which characterize the first half of embryogenesis, the number of CeVPS-32 positive vesicles strongly increases and the size of the vesicles appears smaller. During the second half of embryogenesis, when cells stop dividing and organogenesis proceeds, the CeVPS-32 vesicles are enriched in epithelial tissues such as the intestine, the pharynx and the epidermis. The pattern in pharynx and intestine coincided with the appearance of apical vesicles observed by EM and suggests that they are involved in lumen formation. Immunohistochemical analyses with the anti-CeVPS-32 antibodies revealed that the protein is ubiquitously expressed and detected as early as the 4-cell stage during embryogenesis. Expression is maintained throughout embryonic development, and during larval and adult stages.
• Primary Identifier: Expr8718
• Remark: Picture: Fig 2, Fig 3. The specificity of anti-CeVPS-32 antibodies was confirmed by the almost complete extinction of the signal after inactivation of Cevps-32 by RNA interference and in the Cevps-32(ok1355) null mutant.
• Subcellular Localization: In embryo, CeVPS-32 immunoreactivity was detected in a diffuse cytosolic pattern with a punctiform component characteristic of vesicular localisation. To confirm that CeVPS-32 positive vesicles are endosomes authors looked for colocalization with the endosomal marker CeVPS-27/HGRS-1. Vesicles positive for both CeVPS-32 and CeVPS-27 were detected in all epithelial tissues. However, only a subset (30%, n=606) of vesicles is positive for both markers suggesting that the presence of CeVPS-27 and/or CeVPS-32 correspond to distinct stages of endosomal maturation.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
female genital.	vulva		WBbt:0006748
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00016961	vps-32.1	C56C10.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003