WormMine: ExpressionPattern Expr603

• Pattern: Antibody staining limited to nuclei within pharynx and is detected from beginning of morphogenesis onwards. CEH-22 first detected approx. 330 minutes after fertilization (the lima bean stage) in 11-14 pharyngeal muscle nuclei (probably pharyngeal muscle). At 1.5-fold stage, 14-23 pharyngeal nuclei stain. Cells identified as m3, m4, m5 and m7. In embryos that have completed elongation (pretzel stage), CEH-22 positive nuclei are identified as m3, m4, m5 and m7. Also detected in 6 other pharyngeal nuclei, which are believed to be m1 muscles. After hatching, staining persists in m1, m3, m4, m5 and m7 but absent in m6 and m2.
• Primary Identifier: Expr603
• Remark: Identical staining pattern observed with both antisera (ST::CEH-22 and poly-his::CEH-22 ). Reporter_gene X-gal staining identical to anti-CEH-22 antibodies (Construct contains ~4 kb of ceh-22' -flanking DNA fused to lacZ within the ceh-22 5'-UTR.) This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). see Expr748 for western analysis data.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
first layer pharyngeal muscle cell.	pm1	M1	WBbt:0003744
seventh pharyngeal muscle cell layer	pm7	m7	WBbt:0003721
third pharyngeal muscle cell layer	pm3	M3	WBbt:0003740
fifth pharyngeal muscle cell layer	pm5	M5	WBbt:0003737
fourth pharyngeal muscle cell layer	pm4	M4	WBbt:0003739

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000445	ceh-22	F29F11.5	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015