WormMine: ExpressionPattern Expr1429

• Pattern: Epidermal expression of nhr-23 reporter genes can be achieved with as little as 600 bp of sequence upstream of exon I provided that intron I sequences are also included (construct #4281). However, this construct shows additional expression in nuclei of the pharynx and distal 4-6 nuclei of the intestine, two common sites of ectopic expression. Expression was completely eliminated by removing intron I (construct #4298) and only ectopic (pharynx and intestine) Expression was observed when mostly intron I sequences (construct #4282) were used to drive expression. Reporter gene constructs that begin at least 1.6 kb upstream of exon I and include all of intron I were consistently expressed in epidermal cells and their precursors. Expression in epidermal cells and precursors was clearly evident by the comma stage of embryogenesis and continued through larval stages. Construct #3306 showed the earliest expression, beginning at about the 50-cell stage in one or two unidentified nuclei. Based on the lineage of epidermal cells, it is possible that these early nuclei are Cpa and Caa; however, authors have been unable to confirm this independently.
• Primary Identifier: Expr1429

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
embryonic cell	Caa		WBbt:0006358
embryonic cell	Cpa		WBbt:0006281

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003622	nhr-23	C01H6.5	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018