WormMine: ExpressionPattern Expr10043

• Pattern: All tested strains showed strong enrichment of sup-37 reporter expression within the pharynx beginning at the ~500-cell stage of embryogenesis and continuing throughout larval development and adulthood. In addition, weak-to-modest levels of sup-37 reporter expression were observed in all other cell types. In larval and adult stages, the full-length SUP-37::mCherry was expressed in the pharyngeal muscle groups pm3, pm4, and pm6, but not consistently in other cells of the pharynx. In addition, SUP-37::mCherry expression was faint but detectable in the spermatheca of adult hermaphrodites. Expression of SUP-37::mCherry was not detected in the somatic gonad sheath cells, which also play a role in ovulation.
• Primary Identifier: Expr10043
• Subcellular Localization: Full-length (isoform-a) SUP-37::GFP and SUP- 37::mcherry fusion proteins localize predominantly to nuclei during late stages of embryogenesis. Expression of these constructs was, however, relatively dim and quite variable as compared with the sup-37 transcriptional reporters. Expression of the SUP-37 translational reporters was also occasionally detected in both the cytoplasm and nuclei of early-stage embryos at time points preceding morphogenesis.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
third pharyngeal muscle cell layer	pm3	M3	WBbt:0003740
fourth pharyngeal muscle cell layer	pm4	M4	WBbt:0003739
sixth pharyngeal muscle cell layer	pm6	m6	WBbt:0003724

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006342	sup-37	C01B7.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003