WormMine: ExpressionPattern Expr1866

• Pattern: Expression of reporters confirmed that the main site of synthesis is the pharynx, but it is also expressed in some neurons in the nerve ring, in the ventral chord and in the tail. During embryogenesis, the expression of the constructs is detectable unambiguously only after proliferation has ceased and the embryo has elongated to the two- to three-fold stage. Expression of the reporters does not change substantially from embryogenesis and hatching to larval stages and adults. Expression is strong in several neurons and in pharyngeal cells. The very strong GFP signal detected in the pharynx did not allow the identification of feh-1-expressing cells among those forming this organ. The analysis of nuclear-targeted beta-galactosidase, expressed under the control of the feh-1 gene promoter, clearly identified, in agreement with GFP reporter activity, the nuclei of some extrapharyngeal neurons, as well as neurons of the ventral nerve chord and of the tail. Furthermore, in the procorpus and in the first pharyngeal bulb, the nuclei of m3 and m4 muscle cells are also stained. The staining of the nuclei in the second bulb, where the density is higher, did not unambiguously reveal the identity of feh-1-expressing cells.
• Primary Identifier: Expr1866
• Subcellular Localization: The GFP clearly outlines the shape of the expressing neurons with their processes (e.g. in the ventral nerve chord).

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001410	feh-1	Y54F10AM.2	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020