PHF-5 shows a striking accumulation correlating with the entry of C. elegans embryos in the morphogenetic phase of development. In the first embryonic division stages of C. elegans, no PHF-5::YFP-signal could be detected, while a first expression became visible in the embryonic stage of 100 to 250 cells in the nuclei of two neighboring single cells localized in the central part of the embryos. The expression domain of phf-5::yfp remarkably extends in the following morphogenetic stages of development and shows a dominant distribution highlighting the embryonic pharynx of pretzel-staged embryos and the region of the posterior (rectal) opening. In young larvae, phf-5::yfp expression is restricted to the pharyngeal muscle, to polyploid gut cell nuclei, to body wall muscles, and to the anal muscles. With ongoing development, the PHF-5::YFP-signal is almost restricted to the pharynx of adult C. elegans. --precise ends.
Primary Identifier
Expr2000
Remark
EC610
Subcellular Localization
At higher magnification, the pharyngeal expression shows a slight nuclear predominance while a cytoplasmatic distribution paints the pharyngeal tissue in a homogeneous manner as well does the expression pattern in the body wall muscles. phf-5::yfp distribution in polyploid gut cells is restricted to the cell nuclei.
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5 Anatomy Terms
Definition
Name
Synonym
Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.
The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.
The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.
The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.
The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.
