WormMine: ExpressionPattern Expr3803

• Pattern: Stable transgenic lines expressing the trx-1::gfp translational fusion always resulted in GFP expression in a single pair of amphid neurons. A detailed anatomical analysis identified the ciliated chemosensory neuron pair ASJ-left and -right (ASJL, ASJR) as the cells expressing GFP. GFP fluorescence co-localizing with the fluorescent dye DiI in only one neuron pair, the ventral-most of the fluorescent dye-filling neurons, further confirmed ASJ as the neuron type expressing trx-1 in C. elegans. A detailed sex- and stage-specific analysis of GFP expression showed that GFP fluorescence is first detectable in both male and hermaphrodite in late 3-fold stage embryos, immediately prior to hatching. trx-1::gfp remains expressed in ASJ neurons through all stages, including dauer, until the animal dies. To some extent (in most transgenic animals at all larval stages and in adults, but not in dauers) GFP fluorescence was also detected in the intestine, readily distinguishable from gut autofluorescence, typically in the posterior-most intestinal cells. However, since the number of intestinal cells expressing GFP varied greatly between individuals, the intestinal GFP expression pattern could be due to genetic mosaicism, given that the transgenic animals analyzed in this study carry only extrachromosomal arrays and not integrated transgenes.
• Primary Identifier: Expr3803
• Subcellular Localization: Fluorescence was readily detected in cilia, dendrites, cell bodies (cytoplasm and nucleus) and axons of the ASJ neurons.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJR	lineage name: ABpraaappppa	WBbt:0003885
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJL	lineage name: ABalpppppppa	WBbt:0003886

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00015062	trx-1	B0228.5	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020