WormMine: ExpressionPattern Expr3721

• Pattern: C. elegans whole-mount staining with TBH-1 antibodies labeled a single pair of head interneurons in the lateral ganglion: these neurons were identified as the RICs. Punctate TBH-1 staining was also observed in the gonadal sheath cells of adult hermaphrodites. TBH-1 staining was most prominent in the proximal three pairs of sheath cells, which form a contractile myoepithelium that expels oocytes from the gonad during ovulation.
• Primary Identifier: Expr3721
• Remark: No staining was observed in tbh-1(n3247) mutants.
• Subcellular Localization: Bright staining was observed in RIC synaptic regions, as indicated by a punctate immunofluorescence pattern in the nerve ring, whereas weaker staining was observed in the RIC neuronal processes and cell bodies. By contrast, TBH-1 staining was predominantly localized to the RIC cell bodies of mutants defective in the unc-104 gene, which encodes a neuron-specific kinesin required for the anterograde transport of synaptic vesicles. TBH-1, like the closely related dopamine B-hydroxylase, probably is associated with synaptic vesicles.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Ring interneuron	RICL	lineage name: ABplppaaaapp	WBbt:0003941
Ring interneuron	RICR	lineage name: ABprppaaaapp	WBbt:0003940
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006541	tbh-1	H13N06.6	Caenorhabditis elegans

0 Life Stages