WormMine: ExpressionPattern Expr3088

• Pattern: The reporter was reliably expressed in several easily identified cells including the paired serotonergic neurons NSM and HSN and the dopaminergic PDE postdeirid sensory neurons. NSM processes studded with varicosities were apparent in the isthmus of the pharynx labeled with GFP. The egg-laying neuron HSN normally expresses serotonin only in adulthood, and the reporter was found to be expressed in adult hermaphrodites and sometimes late L4 larvae. Often the HSN processes were apparent extending to vulval muscles and anteriorly within the ventral nerve cord. The bas-1::GFP reporter was also expressed in other neurons in the head, around the nerve ring. The reporter was expressed in probable dorsal and ventral cephalic sensilla neurons CEPD and CEPV; sometimes as many as four processes was observed extending to the tip of the nose. Expression was also seen in the anterior deirid sensory neurons ADE . Less frequently expression was seen in probable ADF and AIM neurons. As many as 12 neurons (6 bilateral pairs) was seen expressing the reporter in the head of young larvae. This includes all the identified serotonergic (NSM, ADF, AIM) and dopaminergic (CEPD, CEPV, ADE) head neurons excepting the unpaired RIH neuron. In a small number of males examined, expression was seen in male-specific serotonergic and dopaminergic neurons, including up to 6 pairs of ray sensory neurons (RNs) in both adults and late L4 larvae . (There are three pairs of serotonergic, and three pairs of dopaminergic RNs among the 18 RNs.) Expression in CP neurons, male-specific ventral cord motoneurons controlling tail curling during mating, was limited and usually weak in the male worms examined. At most three posterior cells showed staining, and usually only one or two posterior cells (CP5, CP6) weakly stained, when expression was present at all. CP staining was never seen in L4 animals, and often none even in male worms expressing GFP strongly in the RNs.
• Primary Identifier: Expr3088

55 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
Neuron, dopaminergic of postderid sensillum	PDER	lineage name: V5R.paaa	WBbt:0004382
Neuron, dopaminergic of postderid sensillum	PDEL	lineage name: V5L.paaa	WBbt:0004383
Pharyngeal neurosecretory motorneuron, contain serotonin	NSMR	lineage name: ABaraapppaav	WBbt:0004446
Pharyngeal neurosecretory motorneuron, contain serotonin	NSML	lineage name: ABaraapapaav	WBbt:0004457
Amphid neuron, prob. chemosensory	ADFL	lineage name: ABalpppppaa	WBbt:0004003
Amphid neuron, prob. chemosensory	ADFR	lineage name: ABpraaappaa	WBbt:0003999
Ring interneuron	AIML	lineage name: ABplpaapppa	WBbt:0003983
Ring interneuron	AIMR	lineage name: ABprpaapppa	WBbt:0003969
Post-embryonic cell of pedigree V6L.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AL	lineage name: V6L.pppapaaa	WBbt:0004030
Post-embryonic cell of pedigree V6R.pppapaaa, male sensory rays, neuron, striated rootlet.	R4AR	lineage name: V6R.pppapaaa	WBbt:0004029
Post-embryonic cell of pedigree V6L.papapaaa, male sensory rays, neuron, striated rootlet.	R2AL	lineage name: R2L.aaa	WBbt:0004042
Post-embryonic cell of pedigree V6R.papapaaa, male sensory rays, neuron, striated rootlet.	R2AR	lineage name: R2R.aaa	WBbt:0004041
Anterior deirid, sensory neuron, dopaminergic	ADEL	lineage name: ABplapaaaapa	WBbt:0004009
Anterior deirid, sensory neuron, dopaminergic	ADER	lineage name: ABprapaaaapa	WBbt:0004007
Cephalic neurons, contain dopamine	CEPDR	lineage name: ABarpapaappa	WBbt:0004937
Cephalic neurons, contain dopamine	CEPVL	lineage name: ABplpaappppa	WBbt:0004933
Cephalic neurons, contain dopamine	CEPVR	lineage name: ABprpaappppa	WBbt:0004931
Cephalic neurons, contain dopamine	CEPDL	lineage name: ABplaaaaappa	WBbt:0004938
Post-embryonic cell of pedigree V5L.pppppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R1BL	lineage name: V5L.pppppapa	WBbt:0004048
Post-embryonic cell of pedigree TL.apappapa, male sensory rays, neuron, darkly staining tip, open to outside.	R7BL	lineage name: TL.apappapa	WBbt:0004001
Post-embryonic cell of pedigree TL.appapapa, male sensory rays, neuron, darkly staining tip, open to outside.	R9BL	lineage name: TL.appapapa	WBbt:0003968
Male sensory rays, neuron, darkly staining tip, open to outside	R9BR	lineage name: TR.appapapa	WBbt:0003966
Male sensory rays, neuron, darkly staining tip, open to outside	R7BR	lineage name: TR.apappapa	WBbt:0004000
Male sensory rays, neuron, darkly staining tip, open to outside	R8BR	lineage name: TR.appaaapa	WBbt:0003988
Post-embryonic cell of pedigree V6L.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BL	lineage name: V6L.ppppaapa	WBbt:0004022
Post-embryonic cell of pedigree V6R.ppppaapa, male sensory rays, neuron, darkly staining tip, open to outside.	R5BR	lineage name: V6R.ppppaapa	WBbt:0004021
Post-embryonic cell of pedigree V6L.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BL	lineage name: V6L.papppapa	WBbt:0004034
Post-embryonic cell of pedigree V6R.papppapa, male sensory rays, neuron, darkly staining tip, open to outside.	R3BR	lineage name: V6R.papppapa	WBbt:0004033

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000239	bas-1	C05D2.4	Caenorhabditis elegans

0 Life Stages