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Expr15957
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mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes. We found that LET-23::mK2 and mNG::LIN-7 overlapped at the basolateral plasma membrane in L3 larvae (from P6.p to P6.pxx). This overlap was more apparent in the differentiated vulval cells (L4). |
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Expr15958
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mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes. |
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Expr10052
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nhr-111 transcripts were detected by real-time qRT- PCR at modest levels in embryos, but decreased progressively during larval development.The nhr-111::gfp reporter was consistently expressed in at least eight pairs of neurons in the head, the sensory PVD neurons of the posterior lateral body wall, the pharynx, intestine (most often in the posterior- and anterior- most cells), the dorsal peri-vulva region of adults (which may be either uterine or vulval cells), and the somatic gonad precursor cells. Among the head neurons was one prominent pair of sensory neurons just posterior to the nerve ring and at least one pair of neurons or support cells that appear to be inner or outer labial sensory cells. We also observed weak and variable expression in a subset of ventral nerve cord motorneurons. The temporal dynamics of nhr-111::gfp were consistent with nhr-111 qRT-PCR results: expression was very bright in the Z1 and Z4 somatic gonad precursor cells in embryos and early L1, but decreased in the developing gonad at later stages and was relatively faint in other cells. nhr-111 transcripts were detected by real-time qRT- PCR at modest levels in embryos, but decreased progressively during larval development.The nhr-111::gfp reporter was consistently expressed in at least eight pairs of neurons in the head, the sensory PVDneurons of the posterior lateral body wall, the pharynx, intestine (most often in the posterior- and anterior- most cells), the dorsal peri-vulva region of adults (which may be either uterine or vulval cells), and the somatic gonad precursor cells. Among the head neurons was one prominent pair of sensory neurons just posterior to the nerve ring and at least one pair of neurons or support cells that appear to be inner or outer labial sensory cells. We also observed weak and variable expression in a subset of ventral nerve cord motorneurons. The temporal dynamics of nhr-111::gfp were consistent with nhr-111 qRT-PCR results: expression was very bright in the Z1 and Z4 somatic gonad precursor cells in embryos and early L1, but decreased in the developing gonad at later stages and was relatively faint in other cells. |
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Picture: Fig 1B, 1C, 1D. |
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Expr8877
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This reporter was expressed in SGPs from shortly after their birth through their divisions in the first larval stage. |
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Expr3085
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After hatching, TRA-1 was still detected in SGPs, but its level and subcellular distribution were distinct in the two sexes. In hermaphrodite L1 SGPs, TRA-1 was largely nuclear, but in male L1 SGPs, TRA-1 was present at a lower level and was uniformly distributed between nucleus and cytoplasm. A similar sexually dimorphic difference was observed in the distribution of GFP::TRA-1 in L1 SGPs. The GFP::TRA-1 reporter partially rescued tra-1(0) mutants, indicating that it produces a functional protein. After the first SGP division, TRA-1 continues to be expressed in hermaphrodite gonads, but is no longer detectable in males. In wild-type XX embryos, TRA-1 was predominantly nuclear in SGPs from formation of the primordium, through embryogenesis, and into the first larval stage. Next, embryos from a fog-2 male/female strain was examined , which produces 50% XX and 50% XO progeny, to determine if TRA-1 is similarly expressed in male SGPs. In 2-fold embryos, most SGPs possessed nuclear TRA-1 (27/30). Therefore, TRA-1 is present in SGP nuclei of both XX hermaphrodite and XO male embryos. |
Expressed in nuclear and cytoplasm. |
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Expr16540
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Animals carrying kpIs106 expressed GVD-1::GFP in various somatic tissues including the somatic gonad, vulva and seam cells. Subcellularly, GVD-1::GFP prominently localized to the nucleus but could be observed in the cytoplasm as well. GVD-1::GFP expression in the somatic gonad started at the L1 stage and persisted until the adult stage; its expression was clearly visible in Z1 and Z4, their progeny and the SGP at the L1, L2 and early L3 stages, respectively. In the vulval lineage, GVD-1::GFP was present in P6.p and its descendants. In late L3 and early L4 larvae, we noticed GVD-1::GFP in the spermathecal/uterine precursors. In adults, GVD-1::GFP could be observed in DTCs, Sh cells and spermatheca. GVD-1::GFP is seen in the developing uterus and vulva at the L3 stage, persisting in the uterus and vulval epithelium (VE) at the L4 stage. |
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Expr11478
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arg-1::GFP is expressed in the head mesodermal cell and tail neurons. Rarely and at very low levels it is expressed in somatic gonadal precursors. |
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Expr12422
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In wild-type animals of both sexes, fkh-6::GFP expression began in the somatic gonadal precursors at about 6 hr after hatching. |
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Expr12421
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In wild-type L1 larvae, rnr::GFP expression first begins in the SGPs approximately 6 hr after hatching, and also occurs in other dividing cells. |
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