WormMine: ExpressionPattern Expr2283

• Pattern: The tagged versions of UNC-5 appear to be expressed in all of the same cell types and with the same timing as previously described for unc-5 transcriptional reporters (see Expr962). Expression of UNC-5::GFP was observed in motor neurons during the comma stage of embryogenesis when axon outgrowth begins, in the ventral nerve cord, the dorsal nerve cord, the commissural motor axons running between the two cords, the excretory canal, and in the DTCs as they migrate toward and then along the dorsal side of the animal. Up-regulation of unc-5 transcriptional reporters was previously shown to be coincident with the beginning of the phase 2 (dorsal to ventral) migration of the DTCs (see Expr962). DTC expression appears slightly delayed when GFP reporters rather than lacZ reporters are used, possibly because of the time it takes for the GFP fluorophore to mature.
• Primary Identifier: Expr2283
• Subcellular Localization: UNC-5::GFP was punctate in all tissues in which it was detected. In neurons, punctate expression is distributed in cell bodies and along axons. Punctate expression was also observed in the veil-like extensions of the following portion of the DTC, which wraps the distal gonad arm. However, UNC-5::GFP appears more abundant around the nucleus at the leading end of the cell and frequently appears most concentrated in the narrow region between the nucleus and the leading edge membrane of the DTC.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
neuron making synapse on an effector (muscle, gland).	motor neuron	motoneuron	WBbt:0005409
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006745	unc-5	B0273.4	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002