WormMine: ExpressionPattern Expr3026

• Pattern: To determine the cellular expression pattern of srf-3, constructs were generated, which fused GFP to the N terminus of SRF-3 and {beta}-galactosidase to the C terminus. Animals transgenic for either of these reporters expressed the fusion genes exclusively in the pharyngeal cells g1 and g2, the lateral seam cells, and the spermatheca. Expression was first visible in the seam cells of late embryos, where it persisted up to the fourth larval stage, but disappeared in adult animals. Expression in the glandular cells g1 and g2 started in L1 and remained visible until adulthood. In sexually mature hermaphrodites, expression was also observed in the spermatheca. In males, a similar pattern of expression was seen in g1, g2, and seam cells. In adult males, a strong expression signal was visible in the vas deferens.
• Primary Identifier: Expr3026
• Subcellular Localization: Intracellular localization of the GFP signal was in a perinuclear staining pattern, consistent with an ER or Golgi apparatus localization of SRF-3.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
Secretory gland cell of the pharynx	Pharyngeal gland cell		WBbt:0005788
That portion of the male reproductive tract, through which sperm are delivered to the proctodeum and then the cloaca in the tail. Thus that portion which is distal to the testis, made of a total of 30 cells.	vas deferens	gon_male_vdef	WBbt:0005337

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00005153	srf-3	M02B1.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021