WormMine: ExpressionPattern Expr2917

• Pattern: Expression of both reporters is observed in all body wall muscles, male sex specific muscles and in the lateral epidermis during post-embryonic development. At the third larval stage, male tail hypodermal expression begins in all dividing Rn.a and Rn.p cells although predominantly in R1.a and R1.p. The strongest expression of the transcriptional reporters is observed in the ray 1 cells. Expression of the transcriptional reporters in other rays is weak and eventually disappears. A similar effect is observed for the translational reporter, which expresses first and most highly on the ray 1 and ray 2 cells. Although the translational reporter is found on all rays at later stages of male tail development, this expression is weak relative to the earlier expression in precursors to rays 1 and 2.
• Primary Identifier: Expr2917
• Remark: Lineage expression: Rn descandents.
• Reporter Gene: [plx-1::gfp] transcriptional and translational fusion constructs. A plx-1 transcriptional gfp reporter was constructed by cloning the 2621 bp sequence immediately 5' to the initiation codon into the multiple cloning site of pPD95_77 to generate plasmid pPD95_77cplx. A plx-1(+) rescuing construct was assembled from multiple PCR fragments encompassing the entire coding sequence of Ce-PLX-1. The 3' portion of the construct comes from the cDNA yk535f1 and contains 739 bp of the 3'UTR. This plx-1(+) cDNA minigene was cloned downstream of the promoter sequence of the pPD95_77cplx transcriptional reporter to obtain the plasmid pZH127. The gfp coding sequence is out of frame in pZH127. The construct contains the full-length plx-1(+) minigene with 2621 bp of sequence immediately 5' to the initiation codon and 739 bp of the 3'UTR sequence. The GFP-encoding portion of pZH127 was put in frame with the PLX-1(+) sequence by fusing it after the PmlI site located four amino acids before the stop codon. For this, a SphI-KpnI fragment was deleted from pZH127, cut with PmlI and re-ligated in combination with a linker sequence into the SphI-KpnI cut pZH127 to obtain the new plx-1 translational reporter plasmid pZH157.

20 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
	copulatory spicule		WBbt:0005312
	spicule retractor muscle		WBbt:0005827
	spicule protractor muscle		WBbt:0005826
specialized muscle type in the posterior of adult male.	longitudinal male muscle	mu_male_long	WBbt:0006909
specialized muscle in the male.	diagonal muscle	dgl	WBbt:0005815
	gubernacular retractor muscle		WBbt:0005825
	gubernacular erector muscle		WBbt:0005824
specialized muscle in the male.	posterior oblique muscle		WBbt:0005823
specialized muscle in the male.	anterior oblique muscle		WBbt:0005822
Male sensory ray 9 hypodermal cell, fuses with hypodermal syncytium	R9_hyp	lineage name: T.appapp, R9.p	WBbt:0004789
Male sensory ray 6 hypodermal cell, fuses with hypodermal syncytium	R6_hyp	lineage name: V6.pppppp, R6.p	WBbt:0004792
Male sensory ray 5 hypodermal cell, fuses with other ray hypodermal cells to form the tail seam	R5_hyp	lineage name: V6.ppppap, R5.p	WBbt:0004793
Male sensory ray 8 hypodermal cell, fuses with hypodermal syncytium	R8_hyp	lineage name: T.appaap, R8.p	WBbt:0004790
Male sensory ray 7 hypodermal cell, fuses with hypodermal syncytium	R7_hyp	lineage name: T.apappp, R7.p	WBbt:0004791
Ray 2 hypodermal cell, fuses with other ray hypodermal cells to form the tail seam	R2_hyp	lineage name: V6.papapp, R2.p	WBbt:0004796
Male sensory ray 4 hypodermal cell, fuses with other ray hypodermal cells to form the tail seam	R4_hyp	lineage name: V6.pppapp, R4.p	WBbt:0004794
Male sensory ray 3 hypodermal cell, fuses with other ray hypodermal cells to form the tail seam	R3_hyp	lineage name: V6.papppp, R3.p	WBbt:0004795
Ray 1 hypodermal cell, fuses with other ray hypodermal cells to form the tail seam.	R1_hyp	lineage name: V5.pppppp, R1.p	WBbt:0004807

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004047	plx-1	Y55F3AL.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024