WormMine: ExpressionPattern Expr1084

• Pattern: Significant GFP fluorescence seen in embryos of living animals (not determined the cell types). In living larvae, however, expression is very low, and in particular no expression was detected during the L3 stage. Hermaphrodites fixed and stained with antibodies to GFP showed consistent LIN-13::GFP accumulation in the nuclei of hyp7 and in seam cells throughout development, including the L3 stage. No reliable expression of LIN-13::GFP observed in the VPCs, although occasionally detected GFP in one or two VPCs in fixed and antibody-stained hermaphrodites.
• Primary Identifier: Expr1084
• Remark: Transgenes expressing either of these LIN-13::GFP hybrid proteins fully rescue the Muv and sterility defects of lin-13(n387) and appear to have identical expression patterns.
• Subcellular Localization: The LIN-13::GFP hybrid proteins localize to the nucleus. In the nucleus, there are also discrete bodies or speckles of fluorescence; other nuclear proteins have been found to localize in such patterns, but in most cases the functional significance of these structures is not clear. Nuclear accumulation observed in other cell types (not characterized in detail, but the expression pattern of LIN-13::GFP seems roughly equivalent to that of lin-13::lacZ).

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
	P6.p hermaphrodite		WBbt:0008125
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite.	P4.p hermaphrodite		WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite.	P3.p hermaphrodite		WBbt:0008112
	P8.p hermaphrodite		WBbt:0008133
	P7.p hermaphrodite		WBbt:0008129

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003002	lin-13	C03B8.4	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002