WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  GFP expression was detected in larvae and adults transgenic worms containing the uggt-1::gfp construct in cells of the nervous system, amphid neurons of the head and the nerve ring; neurons in the dorsal and ventral nerve cords and neurons along the body. Expression was also observed in the phasmid neurons located at the lateral side of the tail. Primary Identifier  Expr9683
Reporter Gene  [uggt-1::GFP] transcriptional fusion. uggt-1 (F48E3.3) promoter sequence was PCR amplified using pDONR P4-P1R vector, obtained from the C. elegans Promoter Library from Open Biosystems (Alabama USA), with a high fidelity polymerase (Hot start KOD, Roche) and cloned into the pPD95.75 polylinker (Addgene) immediately upstream of the GFP coding region to generate the Promoter uggt-1::gfp fusion construct.

7 Anatomy Terms

Definition Name Synonym Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring. ventral nerve cord ventral cord WBbt:0005829
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities. nervous system   WBbt:0005735
neuron with its cell body situated in the tail, posterior to rectum. tail neuron   WBbt:0006759
a bundle of nerve processes that runs along the dorsal mid-line of the animal. dorsal nerve cord dorsal cord WBbt:0006750
neuron of phasmid sensillum phasmid neuron   WBbt:0006753
neuron of the amphid sensillum amphid neuron amphid sensory neuron WBbt:0005394
neuron that has neural processes in the nerve ring. nerve ring neuron   WBbt:0006974

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00018604 uggt-1 F48E3.3 Caenorhabditis elegans

5 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024