WormMine: ExpressionPattern Expr3919

• Pattern: The ALP-1A::GFP is expressed early in muscle development where it first appears during the comma-stage in the characteristic pair of bands that line the lateral surfaces of the embryo, demarcating the nascent body wall muscle cells. Throughout adulthood ALP-1A is most prevalent in the body wall muscle. In addition to the muscle expression, ALP-1A::GFP is expressed in the embryonic hypodermis.
• Primary Identifier: Expr3919
• Subcellular Localization: This muscle-specific localization of ALP-1A::GFP first appears as the muscle cytoskeleton is being established and continues within developing musculature throughout embryogenesis where it is detected both cytoplasmically and in the body wall muscle nuclei. Just before embryonic elongation, ALP-1A::GFP is localized to periodic circumferential fibrils in the hypodermis that persist throughout embryogenesis. This localization pattern is coincident with morphogenetic actin bundles that are required for an actin-myosin-mediated contractile process that drives morphogenesis of the embryo. ALP-1A::GFP localization at these circumferential actin bundles persists throughout morphogenesis during which the fusion protein also localizes to cell junctions in the hypodermal seam cells. Later in development, ALP-1A::GFP expression was detected at the cell-cell junctions of the hypodermal seam cells during larval stages as well.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Epidermal layer.	hypodermis	epidermis	WBbt:0005733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001132	alp-1	T11B7.4	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015