WormMine: ExpressionPattern Expr2305

• Pattern: GFP was detected in larval hyp7 nuclei at levels reduced from those seen in embryonic hypodermis and was not detected in larval body muscle cells. There were some differences between the antibody and GFP results: first, GFP was not reliably detected in the nuclei of ALM and AVM; and second, the nuclei of ventral hypodermal cells had detectable levels of GFP in young (L1-L2 stage) larvae but did not appear to stain with anti-MEC-8 antibodies. The pattern of GFP expression by transgenic embryos carrying mec-8::gfp was very similar to the pattern of MEC-8 expression seen by immunolocalization. GFP was seen in most nuclei at about the 50-cell stage. Just prior to morphogenesis, GFP became brighter in hypodermal nuclei and faded in the nuclei of other cells. During embryonic elongation, hypodermal nuclei exhibited bright GFP fluorescence while other nuclei fluoresced faintly or not at all. The nuclei of hypodermal cells and their precursor cells were marked by staining with anti-LIN-26. The only difference between the GFP expression and the anti-MEC-8 staining was that the faint expression seen in non-hypodermal nuclei carrying mec-8::gfp was not detected with anti-MEC-8 serum. This difference could have been caused by overexpression or perdurance of the MEC-8::GFP fusion protein or by poor antibody sensitivity to low levels of MEC-8.
• Primary Identifier: Expr2305
• Remark: See Expr2304 for antibody staining data.
• Subcellular Localization: nuclei

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003172	mec-8	F46A9.6	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010